Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13288 and 13289 are separated by 507 nucleotides13289 and 13290 are separated by 426 nucleotides13290 and 13291 are separated by 346 nucleotides13291 and 13292 are separated by 124 nucleotides mRNA_4920: 13288 - K01807 rpiA ribose 5-phosphate isomerase A, at 1,311,441 to 1,312,877 13288 mRNA_4921: 13289 - K12156 CYP83A1 cytochrome P450, family 83, subfamily A, polypeptide 1, at 1,313,385 to 1,315,387 13289 mRNA_4922: 13290 - K18670 YAK1 dual specificity protein kinase YAK1, at 1,315,814 to 1,320,351 13290 mRNA_4923: 13291 - K03141 TFIIH1, GTF2H1, TFB1 transcription initiation factor TFIIH subunit 1, at 1,320,698 to 1,323,090 13291 mRNA_4924: 13292 - KOG3012 Uncharacterized conserved protein, at 1,323,215 to 1,324,561 13292
Group Condition mRNA_4920 mRNA_4921 mRNA_4922 mRNA_4923 mRNA_4924
13288 13289 13290 13291 13292
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 -1.7 N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 -0.4 N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.1 N.D. -0.4
growth on oleic acid YNB Oleic Acid N.D. +0.2 -0.3 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -0.3 N.D. -0.3
growth on ricinoleic acid YNB Glucose N.D. +0.0 +0.1 N.D. -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 -0.2 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -0.1 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.8 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.3 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 +0.2 N.D. -0.6
auxotrophy YNB Glucose N.D. +0.2 -0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +0.2 N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 -0.2 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 +0.1 N.D. +0.0
growth on ypd YPD N.D. +0.4 -0.0 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.7 +0.9 N.D. -0.0
auxotrophy Dropout Complete N.D. +0.2 +0.1 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 +0.5 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 +0.0 N.D. -0.1
growth on oleic acid YNB Glucose N.D. +0.4 -0.1 N.D. +0.1
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