Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13289 and 13290 are separated by 426 nucleotides13290 and 13291 are separated by 346 nucleotides13291 and 13292 are separated by 124 nucleotides13292 and 13293 are separated by 286 nucleotides mRNA_4921: 13289 - K12156 CYP83A1 cytochrome P450, family 83, subfamily A, polypeptide 1, at 1,313,385 to 1,315,387 13289 mRNA_4922: 13290 - K18670 YAK1 dual specificity protein kinase YAK1, at 1,315,814 to 1,320,351 13290 mRNA_4923: 13291 - K03141 TFIIH1, GTF2H1, TFB1 transcription initiation factor TFIIH subunit 1, at 1,320,698 to 1,323,090 13291 mRNA_4924: 13292 - KOG3012 Uncharacterized conserved protein, at 1,323,215 to 1,324,561 13292 mRNA_4925: 13293 - ProSiteProfiles-WW/rsp5/WWP domain profile.-PS50020,SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 1,324,848 to 1,328,229 13293
Group Condition mRNA_4921 mRNA_4922 mRNA_4923 mRNA_4924 mRNA_4925
13289 13290 13291 13292 13293
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -1.7 N.D. -0.6 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.8 N.D. +0.4 -1.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.2 N.D. -0.3 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.4 N.D. -0.6 -0.7
growth on ricinoleic acid YNB Glucose +0.0 +0.1 N.D. -0.6 -0.5
growth on oleic acid YNB Oleic Acid +0.2 -0.3 N.D. -0.4 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.2 N.D. -0.6 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.5 N.D. -0.1 -0.7
growth on ypd YPD +0.4 -0.0 N.D. -0.3 -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. +0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 N.D. -0.4 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.3 N.D. -0.3 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.0 N.D. -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.1 N.D. -0.3 +0.5
auxotrophy YNB Glucose +0.2 -0.1 N.D. -0.2 +0.4
growth on oleic acid YNB Glucose +0.4 -0.1 N.D. +0.1 +0.2
auxotrophy Dropout Complete +0.2 +0.1 N.D. -0.1 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 N.D. -0.4 +1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.3 N.D. +0.3 +1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 +0.9 N.D. -0.0 +1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.2 N.D. +0.2 +1.3
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