Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13291 and 13292 are separated by 124 nucleotides13292 and 13293 are separated by 286 nucleotides13293 and 13294 are separated by 87 nucleotides13294 and 13295 are separated by 201 nucleotides mRNA_4923: 13291 - K03141 TFIIH1, GTF2H1, TFB1 transcription initiation factor TFIIH subunit 1, at 1,320,698 to 1,323,090 13291 mRNA_4924: 13292 - KOG3012 Uncharacterized conserved protein, at 1,323,215 to 1,324,561 13292 mRNA_4925: 13293 - ProSiteProfiles-WW/rsp5/WWP domain profile.-PS50020,SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 1,324,848 to 1,328,229 13293 mRNA_4926: 13294 - ProSiteProfiles-Chromo and chromo shadow domain profile.-PS50013, at 1,328,317 to 1,334,558 13294 mRNA_4927: 13295 - HMMPfam-Beta-lactamase-PF00144,SUPERFAMILY--SSF56601, at 1,334,760 to 1,336,928 13295
Group Condition mRNA_4923 mRNA_4924 mRNA_4925 mRNA_4926 mRNA_4927
13291 13292 13293 13294 13295
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.6 -0.7 +0.1 -0.5
growth on oleic acid YNB Oleic Acid N.D. -0.4 -0.3 +0.1 -0.6
growth on ricinoleic acid YNB Glucose N.D. -0.6 -0.5 +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.6 -0.4 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.7 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.9 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 +1.0 +0.1 -1.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.6 -0.5 +0.6 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.2 -0.3 +0.2
growth on oleic acid YNB Glucose N.D. +0.1 +0.2 +0.0 -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.2 -0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -1.1 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 +1.0 -0.2 -0.9
growth on ypd YPD N.D. -0.3 -0.5 +0.2 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +0.5 +0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 +0.5 +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 +0.5 +0.3 +0.0
auxotrophy YNB Glucose N.D. -0.2 +0.4 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 +1.3 -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 +1.3 -0.0 -0.6
auxotrophy Dropout Complete N.D. -0.1 +0.6 +0.1 +0.4
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