Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13297 and 13298 are separated by 326 nucleotides13298 and 13299 are separated by 333 nucleotides13299 and 13300 are separated by 499 nucleotides13300 and 13301 are separated by 338 nucleotides mRNA_4929: 13297 - K01956 carA, CPA1 carbamoyl-phosphate synthase small subunit, at 1,340,791 to 1,342,395 13297 mRNA_4930: 13298 - HMMPfam-Ubiquitin family-PF00240,HMMPfam-BAG domain-PF02179,ProSiteProfiles-Ubiquitin domain profile.-PS50053,ProSiteProfiles-BAG domain profile.-PS51035,SMART-BAG domains, present in regulator of Hsp70 proteins-SM00264,SUPERFAMILY--SSF54236,SUPERFAMILY--SSF63491, at 1,342,722 to 1,344,500 13298 mRNA_4931: 13299 - ProSiteProfiles-BTB domain profile.-PS50097, at 1,344,834 to 1,346,285 13299 mRNA_4932: 13300 - K11547 NDC80, HEC1, TID3 kinetochore protein NDC80, at 1,346,785 to 1,349,520 13300 mRNA_4933: 13301 - K14972 PAXIP1, PTIP PAX-interacting protein 1, at 1,349,859 to 1,354,237 13301
Group Condition mRNA_4929 mRNA_4930 mRNA_4931 mRNA_4932 mRNA_4933
13297 13298 13299 13300 13301
facs on lipid accumulation Sorted High BODIPY Gate -2.8 -0.8 -0.4 N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -3.4 -0.6 -0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -3.3 -0.4 -0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine -4.2 -0.1 +0.3 N.D. +0.0
auxotrophy YNB Glucose -3.8 -0.4 +0.3 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -3.2 -0.4 +0.0 N.D. -0.2
growth on oleic acid YNB Glucose -4.4 +0.5 +0.3 N.D. +0.0
growth on oleic acid YNB Oleic Acid -3.5 -0.0 +0.0 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -3.8 +0.2 +0.5 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -3.9 +0.6 +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -2.6 -0.3 -0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose -3.1 +0.2 +0.1 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -2.4 -0.3 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.2 +0.1 -0.1 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.4 -0.1 -0.5 N.D. +0.0
auxotrophy Dropout Complete -0.6 -0.2 +0.2 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.3 +0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.4 +0.3 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 +0.3 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 +0.2 N.D. -0.1
growth on ypd YPD +0.2 +0.2 +0.4 N.D. -0.1
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