Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13304 and 13305 are separated by 364 nucleotides13305 and 13306 are separated by 695 nucleotides13306 and 13307 are separated by 151 nucleotides13307 and 13308 are separated by 701 nucleotides mRNA_4936: 13304 - HMMPfam-MFS_1 like family-PF12832, at 1,359,714 to 1,362,398 13304 mRNA_4937: 13305 - HMMPfam-Single-strand binding protein family-PF00436,ProSiteProfiles-Single-strand binding (SSB) domain profile.-PS50935,SUPERFAMILY--SSF50249, at 1,362,763 to 1,363,546 13305 mRNA_4938: 13306 - HMMPfam-Protein of unknown function (DUF1749)-PF08538,SUPERFAMILY--SSF53474, at 1,364,242 to 1,365,196 13306 mRNA_4939: 13307 - HMMPfam-RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)-PF00076,ProSiteProfiles-Eukaryotic RNA Recognition Motif (RRM) profile.-PS50102,SMART-RNA recognition motif-SM00360,SUPERFAMILY--SSF54928, at 1,365,348 to 1,366,647 13307 mRNA_4940: 13308 - K06693 PSMD9 26S proteasome non-ATPase regulatory subunit 9, at 1,367,349 to 1,367,987 13308
Group Condition mRNA_4936 mRNA_4937 mRNA_4938 mRNA_4939 mRNA_4940
13304 13305 13306 13307 13308
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. +0.2 -0.3 -0.3
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.1 -0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -0.2 +0.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. -0.2 +0.0 +0.2
growth on ypd YPD +0.4 N.D. -0.4 -0.6 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.3 +0.1 +0.1
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.0 -0.1 -0.0
growth on oleic acid YNB Glucose +0.3 N.D. +0.2 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. +0.7 -0.0 -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. +0.3 -0.2 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. +0.2 +0.4 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. +0.4 +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.0 +0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 +0.3 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.3 -0.0 +0.3
auxotrophy YNB Glucose +0.0 N.D. +0.2 -0.0 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.3 -0.0 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. +0.8 +0.1 +0.0
auxotrophy Dropout Complete -0.0 N.D. +0.3 +0.2 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.7 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +1.0 -0.1 +0.2
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