Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13305 and 13306 are separated by 695 nucleotides13306 and 13307 are separated by 151 nucleotides13307 and 13308 are separated by 701 nucleotides13308 and 13309 are separated by 426 nucleotides mRNA_4937: 13305 - HMMPfam-Single-strand binding protein family-PF00436,ProSiteProfiles-Single-strand binding (SSB) domain profile.-PS50935,SUPERFAMILY--SSF50249, at 1,362,763 to 1,363,546 13305 mRNA_4938: 13306 - HMMPfam-Protein of unknown function (DUF1749)-PF08538,SUPERFAMILY--SSF53474, at 1,364,242 to 1,365,196 13306 mRNA_4939: 13307 - HMMPfam-RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)-PF00076,ProSiteProfiles-Eukaryotic RNA Recognition Motif (RRM) profile.-PS50102,SMART-RNA recognition motif-SM00360,SUPERFAMILY--SSF54928, at 1,365,348 to 1,366,647 13307 mRNA_4940: 13308 - K06693 PSMD9 26S proteasome non-ATPase regulatory subunit 9, at 1,367,349 to 1,367,987 13308 mRNA_4941: 13309 - K12608 CAF16 CCR4-NOT complex subunit CAF16, at 1,368,414 to 1,369,977 13309
Group Condition mRNA_4937 mRNA_4938 mRNA_4939 mRNA_4940 mRNA_4941
13305 13306 13307 13308 13309
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 +0.3 -0.4 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.3 -0.3 -0.4
growth on ypd YPD N.D. -0.4 -0.6 +0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 -0.2 -0.3 -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.3 -0.3 +0.2
growth on oleic acid YNB Glucose N.D. +0.2 -0.3 -0.3 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.1 -0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 -0.0 +0.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 +0.0 +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 +0.1 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.7 -0.0 -0.6 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.3 +0.3 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 +0.4 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.8 +0.1 +0.0 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.3 +0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 -0.0 +0.6 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +1.0 -0.1 +0.2 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 +0.2 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.7 +0.1 +0.1 +0.1
auxotrophy YNB Glucose N.D. +0.2 -0.0 +0.4 +0.5
auxotrophy Dropout Complete N.D. +0.3 +0.2 +0.5 +0.2
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