Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13309 and 13310 are separated by 230 nucleotides13310 and 13311 are separated by 658 nucleotides13311 and 13312 are separated by 320 nucleotides13312 and 13313 are separated by 231 nucleotides mRNA_4941: 13309 - K12608 CAF16 CCR4-NOT complex subunit CAF16, at 1,368,414 to 1,369,977 13309 mRNA_4942: 13310 - Hypothetical Protein, at 1,370,208 to 1,371,359 13310 mRNA_4943: 13311 - K13095 SF1 splicing factor 1, at 1,372,018 to 1,374,079 13311 mRNA_4944: 13312 - KOG1375 Beta tubulin, at 1,374,400 to 1,376,265 13312 mRNA_4945: 13313 - Hypothetical Protein, at 1,376,497 to 1,378,672 13313
Group Condition mRNA_4941 mRNA_4942 mRNA_4943 mRNA_4944 mRNA_4945
13309 13310 13311 13312 13313
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.6 N.D. -1.4 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.3 N.D. -0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.3 N.D. -0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.2 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.2 N.D. -0.2 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.2 N.D. -0.6 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.3 N.D. -0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 N.D. -0.6 +0.5
growth on ricinoleic acid YNB Glucose +0.2 -0.3 N.D. -0.3 +0.0
growth on ypd YPD +0.2 -0.1 N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.3 N.D. -0.3 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.1 N.D. -0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 N.D. -0.0 -0.1
auxotrophy Dropout Complete +0.2 +0.1 N.D. +0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.3 N.D. +0.0 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.2 N.D. +0.2 -0.1
growth on oleic acid YNB Oleic Acid +0.2 +0.3 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.8 N.D. +0.2 +0.2
auxotrophy YNB Glucose +0.5 +0.4 N.D. -0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.2 N.D. +0.2 +0.1
growth on oleic acid YNB Glucose +0.2 +0.5 N.D. +0.4 +0.1
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