Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13324 and 13325 are separated by 578 nucleotides13325 and 13326 are separated by 414 nucleotides13326 and 13327 are separated by 418 nucleotides13327 and 13328 are separated by 508 nucleotides mRNA_4956: 13324 - HMMPfam-SMI1 / KNR4 family (SUKH-1)-PF09346, at 1,398,453 to 1,400,732 13324 mRNA_4957: 13325 - BLAST Ribonuclease P protein subunit p20 [Hypsizygus marmor..., at 1,401,311 to 1,402,401 13325 mRNA_4958: 13326 - K02519 infB, MTIF2 translation initiation factor IF-2, at 1,402,816 to 1,406,002 13326 mRNA_4959: 13327 - K08269 ULK1_2_3, ATG1 serine/threonine-protein kinase ULK/ATG1, at 1,406,421 to 1,410,214 13327 mRNA_4960: 13328 - K00102 dld, LDHD D-lactate dehydrogenase (cytochrome), at 1,410,723 to 1,413,349 13328
Group Condition mRNA_4956 mRNA_4957 mRNA_4958 mRNA_4959 mRNA_4960
13324 13325 13326 13327 13328
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. N.D. -2.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.1 N.D. N.D. -1.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. N.D. -2.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 N.D. N.D. -0.5 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 N.D. N.D. -0.6 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.6 N.D. N.D. -0.1 +0.1
growth on ricinoleic acid YNB Glucose -0.3 N.D. N.D. -0.4 +0.2
auxotrophy Dropout Complete -0.3 N.D. N.D. -0.5 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. N.D. -0.3 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. N.D. -0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.8 N.D. N.D. +0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. N.D. -0.3 +0.3
auxotrophy YNB Glucose -0.5 N.D. N.D. -0.1 +0.3
growth on ypd YPD +0.2 N.D. N.D. -0.4 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid +0.0 N.D. N.D. -0.2 +0.1
growth on oleic acid YNB Glucose +0.0 N.D. N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. +0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. +0.3 +0.4
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