Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13333 and 13334 are separated by 243 nucleotides13334 and 13335 are separated by 874 nucleotides13335 and 13336 are separated by 1123 nucleotides13336 and 13337 are separated by 81 nucleotides mRNA_4965: 13333 - K15156 MED14, RGR1 mediator of RNA polymerase II transcription subunit 14, at 1,421,714 to 1,425,387 13333 mRNA_4966: 13334 - Hypothetical Protein, at 1,425,631 to 1,426,865 13334 mRNA_4967: 13335 - K11593 ELF2C, AGO eukaryotic translation initiation factor 2C, at 1,427,740 to 1,431,350 13335 mRNA_4968: 13336 - KOG4464 Signaling protein RIC-8/synembryn (regulates neurotransmitter secretion), at 1,432,474 to 1,435,003 13336 mRNA_4969: 13337 - K20456 OSBP oxysterol-binding protein 1, at 1,435,085 to 1,439,408 13337
Group Condition mRNA_4965 mRNA_4966 mRNA_4967 mRNA_4968 mRNA_4969
13333 13334 13335 13336 13337
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.7 +0.2 +0.2 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.6 +0.2 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.6 -0.5 +0.0 +0.9 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.6 +0.2 +0.5 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.0 +0.1 +0.4 -0.1
growth on oleic acid YNB Oleic Acid -0.6 +0.0 -0.2 +0.8 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.6 +0.1 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.5 +0.0 +1.1 +0.1
growth on ricinoleic acid YNB Glucose +0.5 -0.2 -0.0 +1.2 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.7 +0.2 +0.0 +0.1
growth on ypd YPD +0.2 +1.0 -0.1 +0.2 +0.4
auxotrophy Dropout Complete +0.7 -0.4 +0.2 +1.2 +0.2
auxotrophy YNB Glucose +0.1 +0.2 +0.1 +1.3 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.3 +0.1 +1.7 +0.2
growth on oleic acid YNB Glucose +0.2 +0.5 +0.1 +1.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +3.1 -0.0 -0.9 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.9 +0.5 +1.3 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 +0.4 +0.2 +1.8 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +3.0 +0.1 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.9 +2.7 +0.1 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +3.6 +0.1 +0.1 +0.2
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