Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13346 and 13347 are separated by 113 nucleotides13347 and 13348 are separated by 428 nucleotides13348 and 13349 are separated by 223 nucleotides13349 and 13350 are separated by 455 nucleotides mRNA_4978: 13346 - K12883 NCBP2, CBP20 nuclear cap-binding protein subunit 2, at 1,465,278 to 1,466,176 13346 mRNA_4979: 13347 - K03575 mutY A/G-specific adenine glycosylase, at 1,466,290 to 1,468,594 13347 mRNA_4980: 13348 - K07432 ALG13 beta-1,4-N-acetylglucosaminyltransferase, at 1,469,023 to 1,469,757 13348 mRNA_4981: 13349 - KOG2117 Uncharacterized conserved protein, at 1,469,981 to 1,471,275 13349 mRNA_4982: 13350 - K17879 NUDT7 peroxisomal coenzyme A diphosphatase NUDT7, at 1,471,731 to 1,473,077 13350
Group Condition mRNA_4978 mRNA_4979 mRNA_4980 mRNA_4981 mRNA_4982
13346 13347 13348 13349 13350
growth on ricinoleic acid YNB Ricinoleic Acid -1.4 -0.0 N.D. N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 -0.9 N.D. N.D. +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 +0.0 N.D. N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.6 -0.1 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.7 -0.2 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.3 N.D. N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy -0.8 +0.0 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.0 N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.2 -0.3 N.D. N.D. +0.1
auxotrophy Dropout Complete -0.2 -0.1 N.D. N.D. -0.1
growth on ypd YPD +0.0 -0.5 N.D. N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.1 N.D. N.D. +0.3
auxotrophy YNB Glucose +0.0 -0.2 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.0 N.D. N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.0 -0.0 N.D. N.D. -0.1
growth on oleic acid YNB Glucose +0.1 -0.1 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.3 N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.1 N.D. N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 -0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.2 +0.3 N.D. N.D. +0.0
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