Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13347 and 13348 are separated by 428 nucleotides13348 and 13349 are separated by 223 nucleotides13349 and 13350 are separated by 455 nucleotides13350 and 13351 are separated by 206 nucleotides mRNA_4979: 13347 - K03575 mutY A/G-specific adenine glycosylase, at 1,466,290 to 1,468,594 13347 mRNA_4980: 13348 - K07432 ALG13 beta-1,4-N-acetylglucosaminyltransferase, at 1,469,023 to 1,469,757 13348 mRNA_4981: 13349 - KOG2117 Uncharacterized conserved protein, at 1,469,981 to 1,471,275 13349 mRNA_4982: 13350 - K17879 NUDT7 peroxisomal coenzyme A diphosphatase NUDT7, at 1,471,731 to 1,473,077 13350 mRNA_4983: 13351 - SUPERFAMILY--SSF48371, at 1,473,284 to 1,476,238 13351
Group Condition mRNA_4979 mRNA_4980 mRNA_4981 mRNA_4982 mRNA_4983
13347 13348 13349 13350 13351
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. N.D. -0.1 -1.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. +0.3 -1.9
growth on ricinoleic acid YNB Glucose -0.0 N.D. N.D. -0.1 -1.1
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. N.D. -0.2 -0.8
auxotrophy YNB Glucose -0.2 N.D. N.D. -0.1 -0.9
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. N.D. +0.1 -1.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. N.D. -0.2 -0.8
auxotrophy Dropout Complete -0.1 N.D. N.D. -0.1 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.2 -0.8
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.1 -0.3
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. +0.1 -0.2
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. +0.1 -0.1
growth on ypd YPD -0.5 N.D. N.D. +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 N.D. N.D. +0.4 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. N.D. -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. +0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. N.D. +0.0 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.1 +0.7
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