Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13348 and 13349 are separated by 223 nucleotides13349 and 13350 are separated by 455 nucleotides13350 and 13351 are separated by 206 nucleotides13351 and 13352 are separated by 173 nucleotides mRNA_4980: 13348 - K07432 ALG13 beta-1,4-N-acetylglucosaminyltransferase, at 1,469,023 to 1,469,757 13348 mRNA_4981: 13349 - KOG2117 Uncharacterized conserved protein, at 1,469,981 to 1,471,275 13349 mRNA_4982: 13350 - K17879 NUDT7 peroxisomal coenzyme A diphosphatase NUDT7, at 1,471,731 to 1,473,077 13350 mRNA_4983: 13351 - SUPERFAMILY--SSF48371, at 1,473,284 to 1,476,238 13351 mRNA_4984: 13352 - HMMPfam-OsmC-like protein-PF02566,SUPERFAMILY--SSF82784, at 1,476,412 to 1,477,119 13352
Group Condition mRNA_4980 mRNA_4981 mRNA_4982 mRNA_4983 mRNA_4984
13348 13349 13350 13351 13352
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 -1.9 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 -1.8 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.2 -0.8 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.2 -0.8 -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 -1.1 -0.0
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.8 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 -1.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 -0.8 -0.4
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.9 +0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 -0.2 -0.5
growth on oleic acid YNB Glucose N.D. N.D. -0.1 -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.0 +0.4 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 -0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.4 +0.5 -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.7 -0.4
growth on ypd YPD N.D. N.D. +0.1 +0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 +0.2 +0.3
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