Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13362 and 13363 are separated by 202 nucleotides13363 and 13364 are separated by 137 nucleotides13364 and 13365 are separated by 226 nucleotides13365 and 13366 are separated by 579 nucleotides mRNA_4994: 13362 - K09568 FKBP1 FK506-binding protein 1, at 1,497,335 to 1,498,309 13362 mRNA_4995: 13363 - Hypothetical Protein, at 1,498,512 to 1,499,346 13363 mRNA_4996: 13364 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 1,499,484 to 1,500,162 13364 mRNA_4997: 13365 - HMMPfam-Fungal specific transcription factor domain-PF04082,SMART-Fungal specific transcription factor domain-SM00906, at 1,500,389 to 1,502,919 13365 mRNA_4998: 13366 - KOG4169 15-hydroxyprostaglandin dehydrogenase and related dehydrogenases, at 1,503,499 to 1,504,533 13366
Group Condition mRNA_4994 mRNA_4995 mRNA_4996 mRNA_4997 mRNA_4998
13362 13363 13364 13365 13366
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -1.1 N.D. -0.4 +0.2
auxotrophy Dropout Complete -0.2 -0.6 N.D. -0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.1 N.D. -0.6 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.2 N.D. -0.5 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -0.6 -0.0
auxotrophy YNB Glucose +0.1 -0.5 N.D. -0.3 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.1 N.D. -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 N.D. -0.5 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.0 N.D. -0.2 -0.2
growth on oleic acid YNB Glucose +0.1 -0.2 N.D. -0.2 -0.1
growth on oleic acid YNB Oleic Acid +0.3 -0.6 N.D. -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.0 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 N.D. +0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.1 N.D. +0.2 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.2 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.2 +0.0 N.D. +0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.0 N.D. +0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.2 N.D. +0.3 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 N.D. +0.0 +0.2
growth on ypd YPD +0.3 +0.6 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.3 N.D. +0.2 +0.3
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