Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13365 and 13366 are separated by 579 nucleotides13366 and 13367 are separated by 367 nucleotides13367 and 13368 are separated by 196 nucleotides13368 and 13369 are separated by 346 nucleotides mRNA_4997: 13365 - HMMPfam-Fungal specific transcription factor domain-PF04082,SMART-Fungal specific transcription factor domain-SM00906, at 1,500,389 to 1,502,919 13365 mRNA_4998: 13366 - KOG4169 15-hydroxyprostaglandin dehydrogenase and related dehydrogenases, at 1,503,499 to 1,504,533 13366 mRNA_4999: 13367 - Hypothetical Protein, at 1,504,901 to 1,505,364 13367 mRNA_5000: 13368 - K16302 CNNM metal transporter CNNM, at 1,505,561 to 1,508,084 13368 mRNA_5001: 13369 - K00649 GNPAT glyceronephosphate O-acyltransferase, at 1,508,431 to 1,511,895 13369
Group Condition mRNA_4997 mRNA_4998 mRNA_4999 mRNA_5000 mRNA_5001
13365 13366 13367 13368 13369
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -0.2 N.D. -0.7 -0.3
growth on oleic acid YNB Glucose -0.2 -0.1 N.D. -0.8 -0.1
auxotrophy YNB Glucose -0.3 -0.0 N.D. -0.4 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 -0.2 N.D. +0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.2 N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -0.0 N.D. -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.2 N.D. -0.5 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.1 N.D. +0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.1 N.D. -0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 N.D. -0.3 -0.0
growth on oleic acid YNB Oleic Acid -0.2 +0.1 N.D. -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.2 N.D. -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.2 N.D. -0.4 +0.0
auxotrophy Dropout Complete -0.2 -0.1 N.D. -0.0 +0.1
growth on ricinoleic acid YNB Glucose +0.0 +0.1 N.D. +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.1 N.D. +0.3 -0.2
growth on ypd YPD +0.1 +0.2 N.D. -0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.1 N.D. -0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.0 N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.3 N.D. -0.4 +0.3
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