Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13368 and 13369 are separated by 346 nucleotides13369 and 13370 are separated by 1709 nucleotides13370 and 13371 are separated by 614 nucleotides13371 and 13372 are separated by 222 nucleotides mRNA_5000: 13368 - K16302 CNNM metal transporter CNNM, at 1,505,561 to 1,508,084 13368 mRNA_5001: 13369 - K00649 GNPAT glyceronephosphate O-acyltransferase, at 1,508,431 to 1,511,895 13369 mRNA_5002: 13370 - K02981 RP-S2e, RPS2 small subunit ribosomal protein S2e, at 1,513,605 to 1,514,626 13370 mRNA_5003: 13371 - Hypothetical Protein, at 1,515,241 to 1,515,824 13371 mRNA_5004: 13372 - K12848 SNU23 U4/U6.U5 tri-snRNP component SNU23, at 1,516,047 to 1,517,117 13372
Group Condition mRNA_5000 mRNA_5001 mRNA_5002 mRNA_5003 mRNA_5004
13368 13369 13370 13371 13372
growth on oleic acid YNB Glucose -0.8 -0.1 N.D. +0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 -0.3 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.1 N.D. -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.0 N.D. -0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.0 N.D. +0.0 N.D.
auxotrophy YNB Glucose -0.4 +0.0 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.3 N.D. -0.3 N.D.
growth on ypd YPD -0.2 +0.0 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.2 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.0 N.D. -0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.1 -0.1 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.2 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.2 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.2 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.1 N.D. +0.3 N.D.
auxotrophy Dropout Complete -0.0 +0.1 N.D. +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.2 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.0 N.D. +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 N.D. +0.3 N.D.
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