Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13370 and 13371 are separated by 614 nucleotides13371 and 13372 are separated by 222 nucleotides13372 and 13373 are separated by 5684 nucleotides13373 and 13374 are separated by 269 nucleotides mRNA_5002: 13370 - K02981 RP-S2e, RPS2 small subunit ribosomal protein S2e, at 1,513,605 to 1,514,626 13370 mRNA_5003: 13371 - Hypothetical Protein, at 1,515,241 to 1,515,824 13371 mRNA_5004: 13372 - K12848 SNU23 U4/U6.U5 tri-snRNP component SNU23, at 1,516,047 to 1,517,117 13372 mRNA_5005: 13373 - K10956 SEC61A protein transport protein SEC61 subunit alpha, at 1,522,802 to 1,524,731 13373 mRNA_5006: 13374 - K20299 VPS53 vacuolar protein sorting-associated protein 53, at 1,525,001 to 1,528,336 13374
Group Condition mRNA_5002 mRNA_5003 mRNA_5004 mRNA_5005 mRNA_5006
13370 13371 13372 13373 13374
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. -1.8 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. -1.3 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 N.D. -0.7 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 N.D. -0.2 -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 N.D. -0.6 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 N.D. -0.4 +0.1
growth on ypd YPD N.D. -0.1 N.D. -0.4 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 N.D. -0.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Glucose N.D. -0.2 N.D. +0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 N.D. +0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. +0.0 -0.2
growth on oleic acid YNB Glucose N.D. +0.0 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. +0.2 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. +0.4 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 N.D. +0.6 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. +0.5 +0.0
auxotrophy YNB Glucose N.D. +0.0 N.D. +0.7 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.4 N.D. +0.2 +0.1
auxotrophy Dropout Complete N.D. +0.1 N.D. +0.5 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. +0.2 +0.4
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