Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13378 and 13379 are separated by 171 nucleotides13379 and 13380 are separated by 218 nucleotides13380 and 13381 are separated by 232 nucleotides13381 and 13382 are separated by 1189 nucleotides mRNA_5010: 13378 - K17576 PPP1R37, LRRC68 protein phosphatase 1 regulatory subunit 37, at 1,534,443 to 1,540,178 13378 mRNA_5011: 13379 - BLAST transmembrane protein, putative [Rhizoctonia solani A..., at 1,540,350 to 1,542,527 13379 mRNA_5012: 13380 - K01886 QARS, glnS glutaminyl-tRNA synthetase, at 1,542,746 to 1,545,688 13380 mRNA_5013: 13381 - BLAST regulatory protein wetA [Paracoccidioides lutzii ..., at 1,545,921 to 1,548,060 13381 mRNA_5014: 13382 - K01621 xfp, xpk xylulose-5-phosphate/fructose-6-phosphate phosphoketolase, at 1,549,250 to 1,553,078 13382
Group Condition mRNA_5010 mRNA_5011 mRNA_5012 mRNA_5013 mRNA_5014
13378 13379 13380 13381 13382
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -1.1 -0.9 +0.2 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.0 -1.8 +0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.9 -1.0 +0.4 -0.2
growth on ricinoleic acid YNB Glucose +0.5 -0.5 -0.9 +0.1 -0.4
growth on ypd YPD +0.2 -0.1 -0.8 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.6 +0.2 -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 -0.2 -0.4 +0.1
growth on oleic acid YNB Oleic Acid +0.4 -0.4 -0.3 +0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.4 -0.4 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.1 +0.1 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.2 -0.2 +0.0 +0.6
auxotrophy Dropout Complete +0.4 -0.2 -0.1 +0.5 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 +0.1 -0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 +0.2 -0.6 +0.5 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.4 +0.2 +0.5 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.3 +0.6 +0.5 -0.1
growth on oleic acid YNB Glucose +0.5 -0.2 +0.1 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 -0.0 +0.6 +0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 +0.4 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 +0.2 +0.1 +0.4
auxotrophy YNB Glucose +0.6 -0.2 +0.1 +0.7 -0.4
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