Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13388 and 13389 are separated by 190 nucleotides13389 and 13390 are separated by 241 nucleotides13390 and 13391 are separated by 240 nucleotides13391 and 13392 are separated by 491 nucleotides mRNA_5020: 13388 - K20178 VPS8 vacuolar protein sorting-associated protein 8, at 1,563,647 to 1,569,301 13388 mRNA_5021: 13389 - K14347 SLC10A7, P7 solute carrier family 10 (sodium/bile acid cotransporter), member 7, at 1,569,492 to 1,571,260 13389 mRNA_5022: 13390 - Hypothetical Protein, at 1,571,502 to 1,572,574 13390 mRNA_5023: 13391 - K17866 DPH2 diphthamide biosynthesis protein 2, at 1,572,815 to 1,575,164 13391 mRNA_5024: 13392 - K11124 SMG6, EST1A protein SMG6, at 1,575,656 to 1,580,280 13392
Group Condition mRNA_5020 mRNA_5021 mRNA_5022 mRNA_5023 mRNA_5024
13388 13389 13390 13391 13392
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 -0.5 -0.1 +0.3 -2.3
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.0 -0.4 -0.3 -0.3
growth on oleic acid YNB Glucose -0.5 -0.0 -0.6 -0.3 +0.3
auxotrophy YNB Glucose -0.3 +0.2 -0.4 -0.8 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.4 -0.3 +0.2 +0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -1.4 +0.3 +0.6 -0.7 +0.2
growth on ypd YPD +0.3 +0.0 -0.5 -0.1 -0.7
auxotrophy YNB Glucose 75mM Arginine -0.8 +0.4 -0.3 -0.4 +0.4
growth on oleic acid YNB Oleic Acid -0.9 +0.1 -0.2 -0.4 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.5 +0.0 -1.4 +0.5
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 -0.0 +0.0 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -1.2 +0.8 +0.3 -0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 -0.2 -0.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.5 -0.2 -0.3 -1.1
auxotrophy Dropout Complete -0.1 +0.0 -0.5 -0.0 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 -0.4 +0.1 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.5 -0.1 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.0 +0.0 -0.1 -0.3 +0.4
growth on ricinoleic acid YNB Glucose +1.0 +0.4 -0.3 -0.5 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.1 +0.1 +0.5 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.1 +0.9 +0.1 +0.3
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