Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13391 and 13392 are separated by 491 nucleotides13392 and 13393 are separated by 433 nucleotides13393 and 13394 are separated by 186 nucleotides13394 and 13395 are separated by 544 nucleotides mRNA_5023: 13391 - K17866 DPH2 diphthamide biosynthesis protein 2, at 1,572,815 to 1,575,164 13391 mRNA_5024: 13392 - K11124 SMG6, EST1A protein SMG6, at 1,575,656 to 1,580,280 13392 mRNA_5025: 13393 - BLAST HECT-type ubiquitin ligase-interacting protein creD [..., at 1,580,714 to 1,583,108 13393 mRNA_5026: 13394 - K01698 hemB, ALAD porphobilinogen synthase, at 1,583,295 to 1,584,679 13394 mRNA_5027: 13395 - HMMPfam-Cryptococcal mannosyltransferase 1-PF11735, at 1,585,224 to 1,587,309 13395
Group Condition mRNA_5023 mRNA_5024 mRNA_5025 mRNA_5026 mRNA_5027
13391 13392 13393 13394 13395
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -2.3 -0.3 N.D. -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -1.1 +0.3 N.D. -0.8
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.2 +0.1 N.D. -1.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.4 +0.5 -0.6 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.3 -0.3 N.D. -0.3
auxotrophy YNB Glucose -0.8 +0.3 -0.2 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.4 -0.4 N.D. -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 +0.2 -0.1 N.D. -0.3
auxotrophy Dropout Complete -0.0 +0.4 -0.3 N.D. -0.8
growth on oleic acid YNB Oleic Acid -0.4 +0.6 -0.2 N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.1 -0.2 N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.5 +0.7 -0.5 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.1 -0.4 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.6 -0.1 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.5 -0.2 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 -0.2 N.D. -0.1
growth on ypd YPD -0.1 -0.7 +0.2 N.D. +0.7
growth on oleic acid YNB Glucose -0.3 +0.3 +0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.3 -0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.4 -0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 +0.9 -0.5 N.D. -0.1
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