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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
13397 and 13398 are separated by 103 nucleotides
13398 and 13399 are separated by 530 nucleotides
13399 and 13400 are separated by 502 nucleotides
13400 and 13401 are separated by 893 nucleotides
mRNA_5029: 13397 - HMMPfam-Ornithine decarboxylase antizyme-PF02100,SUPERFAMILY--SSF55729, at 1,591,057 to 1,592,630
13397
mRNA_5030: 13398 - K01889 FARSA, pheS phenylalanyl-tRNA synthetase alpha chain, at 1,592,734 to 1,594,516
13398
mRNA_5031: 13399 - HMMPfam-PAS fold-PF00989,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,ProSiteProfiles-PAS repeat profile.-PS50112,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF55785, at 1,595,047 to 1,597,304
13399
mRNA_5032: 13400 - Hypothetical Protein, at 1,597,807 to 1,600,574
13400
mRNA_5033: 13401 - KOG4436 Predicted GTPase activator NB4S/EVI5 (contains TBC domain)/Calmodulin-binding protein Pollux (contains PTB and TBC domains), at 1,601,468 to 1,605,781
13401
Group
Condition
mRNA
_5029
mRNA
_5030
mRNA
_5031
mRNA
_5032
mRNA
_5033
13397
13398
13399
13400
13401
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
N.D.
-2.6
-0.3
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.2
N.D.
-2.2
+0.2
-0.5
ypd buoyancy
40 hrs No Buoyancy Separation
-0.5
N.D.
-0.4
-1.7
+0.4
growth on oleic acid
YNB Oleic Acid
-0.1
N.D.
-0.7
-0.1
-0.1
growth on ricinoleic acid
YNB Glucose
-0.1
N.D.
-0.3
-0.0
-0.5
auxotrophy
YNB Glucose
+0.1
N.D.
-0.4
+0.0
-0.0
growth on ypd
YPD
-0.1
N.D.
-0.0
-0.2
+0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.1
N.D.
-0.3
+0.3
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
N.D.
+0.4
+0.0
-0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.1
N.D.
-0.2
+0.2
-0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.0
N.D.
-0.0
+0.2
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
N.D.
+0.0
+0.3
-0.1
ypd buoyancy
40 hrs High Buoyancy
-0.1
N.D.
+0.1
-0.1
+0.4
auxotrophy
Dropout Complete
+0.2
N.D.
+0.0
+0.1
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
N.D.
+0.1
+0.2
-0.0
ypd buoyancy
40 hrs Low Buoyancy
+0.1
N.D.
+0.3
-0.1
+0.1
growth on oleic acid
YNB Glucose
+0.0
N.D.
-0.2
+0.8
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
+0.1
N.D.
+0.2
+0.1
+0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
N.D.
-0.0
+0.7
+0.2
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
N.D.
+0.3
+0.4
+0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
N.D.
+0.6
+0.3
+0.0
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