Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13397 and 13398 are separated by 103 nucleotides13398 and 13399 are separated by 530 nucleotides13399 and 13400 are separated by 502 nucleotides13400 and 13401 are separated by 893 nucleotides mRNA_5029: 13397 - HMMPfam-Ornithine decarboxylase antizyme-PF02100,SUPERFAMILY--SSF55729, at 1,591,057 to 1,592,630 13397 mRNA_5030: 13398 - K01889 FARSA, pheS phenylalanyl-tRNA synthetase alpha chain, at 1,592,734 to 1,594,516 13398 mRNA_5031: 13399 - HMMPfam-PAS fold-PF00989,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,ProSiteProfiles-PAS repeat profile.-PS50112,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF55785, at 1,595,047 to 1,597,304 13399 mRNA_5032: 13400 - Hypothetical Protein, at 1,597,807 to 1,600,574 13400 mRNA_5033: 13401 - KOG4436 Predicted GTPase activator NB4S/EVI5 (contains TBC domain)/Calmodulin-binding protein Pollux (contains PTB and TBC domains), at 1,601,468 to 1,605,781 13401
Group Condition mRNA_5029 mRNA_5030 mRNA_5031 mRNA_5032 mRNA_5033
13397 13398 13399 13400 13401
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -2.6 -0.3 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -2.2 +0.2 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. -0.4 -1.7 +0.4
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.7 -0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.3 -0.0 -0.5
auxotrophy YNB Glucose +0.1 N.D. -0.4 +0.0 -0.0
growth on ypd YPD -0.1 N.D. -0.0 -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.3 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. +0.4 +0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.2 +0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. -0.0 +0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.0 +0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 -0.1 +0.4
auxotrophy Dropout Complete +0.2 N.D. +0.0 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.1 +0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.3 -0.1 +0.1
growth on oleic acid YNB Glucose +0.0 N.D. -0.2 +0.8 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.2 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.0 +0.7 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. +0.3 +0.4 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.6 +0.3 +0.0
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