Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13409 and 13410 are separated by 186 nucleotides13410 and 13411 are separated by 334 nucleotides13411 and 13412 are separated by 345 nucleotides13412 and 13413 are separated by 435 nucleotides mRNA_5041: 13409 - K01077 E3.1.3.1, phoA, phoB alkaline phosphatase, at 1,617,376 to 1,619,999 13409 mRNA_5042: 13410 - K11987 PTGS2, COX2 prostaglandin-endoperoxide synthase 2, at 1,620,186 to 1,624,463 13410 mRNA_5043: 13411 - PIRSF--PIRSF022976, at 1,624,798 to 1,625,839 13411 mRNA_5044: 13412 - K04717 SGPP2 sphingosine-1-phosphate phosphotase 2, at 1,626,185 to 1,628,118 13412 mRNA_5045: 13413 - K01111 E3.1.3.68 2-deoxyglucose-6-phosphatase, at 1,628,554 to 1,629,804 13413
Group Condition mRNA_5041 mRNA_5042 mRNA_5043 mRNA_5044 mRNA_5045
13409 13410 13411 13412 13413
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.4 +0.2 -0.8 +0.1
growth on oleic acid YNB Glucose -0.1 -0.1 -0.3 +0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.6 +0.3 -0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 +0.1 -0.0 -0.4
growth on oleic acid YNB Oleic Acid +0.1 -0.1 -0.1 +0.2 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 -0.0 +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.1 +0.1 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.1 -0.5 +0.0 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.4 +0.2 -0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.1 -0.6 -0.2 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.2 -0.3 +0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.2 -0.0 -0.2 +0.4
growth on ricinoleic acid YNB Glucose -0.1 -0.0 -0.4 -0.1 +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 -0.7 +0.3 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.1 -0.1 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.2 +0.1 +0.1 +0.2
auxotrophy Dropout Complete +0.1 +0.2 -0.1 +0.2 +0.6
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.5 +0.1 -0.1 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.4 +0.1 +0.0 +0.6
auxotrophy YNB Glucose +0.3 +0.3 +0.0 -0.1 +0.7
growth on ypd YPD +0.3 -0.0 +0.4 -0.1 +0.7
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