Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13416 and 13417 are separated by 192 nucleotides13417 and 13418 are separated by 246 nucleotides13418 and 13419 are separated by 259 nucleotides13419 and 13420 are separated by 184 nucleotides mRNA_5048: 13416 - HMMPfam-Animal haem peroxidase-PF03098,ProSiteProfiles-Animal heme peroxidase superfamily profile.-PS50292,SUPERFAMILY--SSF48113, at 1,632,775 to 1,633,418 13416 mRNA_5049: 13417 - HMMPfam-Cytochrome P450-PF00067,SUPERFAMILY--SSF48264, at 1,633,611 to 1,634,725 13417 mRNA_5050: 13418 - Hypothetical Protein, at 1,634,972 to 1,635,708 13418 mRNA_5051: 13419 - KOG1382 Multiple inositol polyphosphate phosphatase, at 1,635,968 to 1,637,866 13419 mRNA_5052: 13420 - Hypothetical Protein, at 1,638,051 to 1,638,624 13420
Group Condition mRNA_5048 mRNA_5049 mRNA_5050 mRNA_5051 mRNA_5052
13416 13417 13418 13419 13420
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.1 -0.4 -1.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. -0.3 -0.5 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.1 -0.5 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 N.D. -0.2 +0.6 -1.9
ypd buoyancy 40 hrs High Buoyancy -0.7 N.D. +0.1 +0.3 -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. +0.3 +0.3 -1.1
growth on oleic acid YNB Glucose +0.1 N.D. -0.2 -0.3 +0.3
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.2 -0.3 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. +0.1 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.2 -0.2 -0.3
auxotrophy Dropout Complete -0.3 N.D. -0.2 +0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +0.0 +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.1 +0.3 +0.2
auxotrophy YNB Glucose -0.1 N.D. +0.1 +0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. -0.1 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 N.D. -0.4 -0.1 +0.5
growth on ypd YPD +0.9 N.D. -0.0 +0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +1.2 N.D. -0.1 -0.3 +0.3
growth on ricinoleic acid YNB Glucose +1.2 N.D. -0.1 -0.4 +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.6 N.D. +0.1 +0.0 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +1.5 N.D. -0.2 -0.2 +0.4
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