Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13429 and 13430 are separated by 68 nucleotides13430 and 13431 are separated by 308 nucleotides13431 and 13432 are separated by 247 nucleotides13432 and 13433 are separated by 214 nucleotides mRNA_5061: 13429 - K11414 SIRT4, SIR2L4 NAD-dependent deacetylase sirtuin 4, at 1,661,158 to 1,662,498 13429 mRNA_5062: 13430 - HMMPfam-Zinc-binding-PF12907,SUPERFAMILY--SSF118359, at 1,662,567 to 1,663,555 13430 mRNA_5063: 13431 - K10301 FBXO21 F-box protein 21, at 1,663,864 to 1,665,707 13431 mRNA_5064: 13432 - K00286 proC pyrroline-5-carboxylate reductase, at 1,665,955 to 1,667,428 13432 mRNA_5065: 13433 - HMMPfam-Mitotic checkpoint regulator, MAD2B-interacting-PF10253, at 1,667,643 to 1,669,072 13433
Group Condition mRNA_5061 mRNA_5062 mRNA_5063 mRNA_5064 mRNA_5065
13429 13430 13431 13432 13433
growth on ricinoleic acid YNB Glucose -0.6 +0.1 -0.2 N.D. -0.7
auxotrophy Dropout Complete -0.1 -0.2 +0.0 N.D. -1.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.0 +0.1 N.D. -1.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.3 -0.8 N.D. -1.1
auxotrophy YNB Glucose +0.0 -0.0 +0.1 N.D. -1.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.1 +0.0 N.D. -1.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.1 +0.2 N.D. -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.1 -0.0 N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.0 +0.2 N.D. -1.0
growth on oleic acid YNB Glucose +0.0 -0.2 -0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.1 +0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.5 +0.4 N.D. -1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.6 +0.2 N.D. -1.0
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.2 -0.3 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.1 +0.5 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.6 +0.1 N.D. -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.4 +0.4 N.D. -0.9
growth on oleic acid YNB Oleic Acid +0.2 -0.2 +0.1 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.4 +0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.3 -0.2 N.D. +0.4
growth on ypd YPD +0.1 -0.0 +0.1 N.D. +0.7
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