Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13441 and 13442 are separated by 209 nucleotides13442 and 13443 are separated by 195 nucleotides13443 and 13444 are separated by 176 nucleotides13444 and 13445 are separated by 289 nucleotides mRNA_5073: 13441 - K12843 PRPF3, PRP3 U4/U6 small nuclear ribonucleoprotein PRP3, at 1,684,335 to 1,686,538 13441 mRNA_5074: 13442 - KOG1426 FOG RCC1 domain, at 1,686,748 to 1,688,020 13442 mRNA_5075: 13443 - K00958 sat, met3 sulfate adenylyltransferase, at 1,688,216 to 1,690,274 13443 mRNA_5076: 13444 - K00588 E2.1.1.104 caffeoyl-CoA O-methyltransferase, at 1,690,451 to 1,691,745 13444 mRNA_5077: 13445 - KOG1663 O-methyltransferase, at 1,692,035 to 1,693,349 13445
Group Condition mRNA_5073 mRNA_5074 mRNA_5075 mRNA_5076 mRNA_5077
13441 13442 13443 13444 13445
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -2.3 +0.0 -1.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -1.3 -0.8 -1.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -1.4 -0.1 -1.5
growth on ricinoleic acid YNB Glucose N.D. +0.0 -2.6 -0.0 -0.2
auxotrophy YNB Glucose N.D. +0.3 -2.4 +0.0 -0.6
growth on oleic acid YNB Glucose N.D. +0.1 -2.2 -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -2.1 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.6 -0.4 -1.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 -1.9 -0.0 -0.5
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.2 -0.5 -1.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 -0.2 -0.0 -0.9
growth on ypd YPD N.D. -0.0 -0.0 -0.3 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -3.0 +0.2 +2.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.3 -0.7 +0.1
auxotrophy Dropout Complete N.D. +0.0 -0.1 -0.2 -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.3 +0.3 +0.1 -1.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.1 +0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.2 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 -1.5 +0.2 +2.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 -0.5 +0.4 +1.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 +1.1 +0.4 +0.1
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