Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13446 and 13447 are separated by 148 nucleotides13447 and 13448 are separated by 204 nucleotides13448 and 13449 are separated by 204 nucleotides13449 and 13450 are separated by 706 nucleotides mRNA_5078: 13446 - K01870 IARS, ileS isoleucyl-tRNA synthetase, at 1,694,035 to 1,697,967 13446 mRNA_5079: 13447 - HMMPfam-TRAF-type zinc finger-PF02176,ProSiteProfiles-Zinc finger TRAF-type profile.-PS50145, at 1,698,116 to 1,699,686 13447 mRNA_5080: 13448 - K12486 SMAP stromal membrane-associated protein, at 1,699,891 to 1,701,497 13448 mRNA_5081: 13449 - K10396 KIF5 kinesin family member 5, at 1,701,702 to 1,705,465 13449 mRNA_5082: 13450 - K10696 BRE1 E3 ubiquitin-protein ligase BRE1, at 1,706,172 to 1,709,244 13450
Group Condition mRNA_5078 mRNA_5079 mRNA_5080 mRNA_5081 mRNA_5082
13446 13447 13448 13449 13450
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 -3.5 -0.3 -2.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 -1.6 -0.0 -2.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.7 -0.4 -0.1 -1.9
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.1 -0.1 -0.2 -0.3
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.2 -0.2 -1.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.4 -0.8 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.7 -0.3 -0.0
auxotrophy YNB Glucose N.D. -0.4 +0.1 -0.4 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.0 +0.1 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.0 -0.1 -0.7
growth on oleic acid YNB Oleic Acid N.D. +0.3 -0.5 -0.4 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 +0.4 +0.3 -0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.6 -0.5 -0.3 +0.3
auxotrophy Dropout Complete N.D. -0.1 +0.3 +0.2 -0.3
growth on ypd YPD N.D. -0.4 +0.1 -0.1 +0.6
growth on oleic acid YNB Glucose N.D. +0.4 -0.1 -0.2 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 +0.3 +0.5 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 +1.2 -0.6 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.5 -0.6 +1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 +0.9 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +1.6 -0.3 -0.2
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