Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13452 and 13453 are separated by 461 nucleotides13453 and 13454 are separated by 445 nucleotides13454 and 13455 are separated by 584 nucleotides13455 and 13456 are separated by 1037 nucleotides mRNA_5084: 13452 - Hypothetical Protein, at 1,712,081 to 1,713,113 13452 mRNA_5085: 13453 - K03114 SWE1 mitosis inhibitor protein kinase SWE1, at 1,713,575 to 1,718,453 13453 mRNA_5086: 13454 - Hypothetical Protein, at 1,718,899 to 1,719,393 13454 mRNA_5087: 13455 - K06890 K06890 uncharacterized protein, at 1,719,978 to 1,721,212 13455 mRNA_5088: 13456 - BLAST cytochrome P450 [Coprinopsis cinerea okayama7#130], at 1,722,250 to 1,723,721 13456
Group Condition mRNA_5084 mRNA_5085 mRNA_5086 mRNA_5087 mRNA_5088
13452 13453 13454 13455 13456
facs on lipid accumulation Sorted High BODIPY Gate -2.0 -0.6 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -1.8 N.D. +0.0 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.8 N.D. +0.2 -0.4
growth on ricinoleic acid YNB Glucose -0.1 -0.9 N.D. +0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -1.1 N.D. -0.2 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.8 N.D. -0.1 +0.5
auxotrophy YNB Glucose +0.1 -0.9 N.D. -0.3 +0.6
auxotrophy Dropout Complete +0.3 -0.7 N.D. -0.2 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.1 N.D. +0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 N.D. +0.1 -0.1
growth on ypd YPD -0.1 +0.1 N.D. -0.0 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.3 N.D. +0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.6 N.D. +0.5 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.5 N.D. +0.1 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.1 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.8 +0.3 N.D. +0.2 +0.5
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.1 N.D. +0.5 +0.0
growth on oleic acid YNB Glucose -0.1 +0.2 N.D. +0.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +2.1 -0.8 N.D. -0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.5 N.D. +0.5 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.6 -0.2 N.D. +0.4 +0.4
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