Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13462 and 13463 are separated by 136 nucleotides13463 and 13464 are separated by 1187 nucleotides13464 and 13465 are separated by 297 nucleotides13465 and 13466 are separated by 882 nucleotides mRNA_5094: 13462 - K10259 MET30 F-box and WD-40 domain protein MET30, at 1,733,465 to 1,736,154 13462 mRNA_5095: 13463 - K03106 SRP54, ffh signal recognition particle subunit SRP54, at 1,736,291 to 1,738,860 13463 mRNA_5096: 13464 - K11292 SUPT6H, SPT6 transcription elongation factor SPT6, at 1,740,048 to 1,744,775 13464 mRNA_5097: 13465 - Hypothetical Protein, at 1,745,073 to 1,746,754 13465 mRNA_5098: 13466 - K08065 NFYB nuclear transcription Y subunit beta, at 1,747,637 to 1,748,369 13466
Group Condition mRNA_5094 mRNA_5095 mRNA_5096 mRNA_5097 mRNA_5098
13462 13463 13464 13465 13466
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.6 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.2 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.1 -0.5
auxotrophy Dropout Complete N.D. N.D. N.D. -0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.4 +0.1
growth on ypd YPD N.D. N.D. N.D. +0.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.1 -0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.3 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.3 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.1 +0.2
auxotrophy YNB Glucose N.D. N.D. N.D. -0.2 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.0 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 +0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.0 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.3 +1.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.3 +1.6
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