Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13480 and 13481 are separated by 226 nucleotides13481 and 13482 are separated by 296 nucleotides13482 and 13483 are separated by 233 nucleotides13483 and 13484 are separated by 439 nucleotides mRNA_5112: 13480 - KOG0872 Sterol C5 desaturase, at 1,789,147 to 1,790,624 13480 mRNA_5113: 13481 - KOG1441 Glucose-6-phosphate/phosphate and phosphoenolpyruvate/phosphate antiporter, at 1,790,851 to 1,792,363 13481 mRNA_5114: 13482 - K14820 BRX1, BRIX1 ribosome biogenesis protein BRX1, at 1,792,660 to 1,793,898 13482 mRNA_5115: 13483 - Hypothetical Protein, at 1,794,132 to 1,795,760 13483 mRNA_5116: 13484 - K00721 DPM1 dolichol-phosphate mannosyltransferase, at 1,796,200 to 1,797,488 13484
Group Condition mRNA_5112 mRNA_5113 mRNA_5114 mRNA_5115 mRNA_5116
13480 13481 13482 13483 13484
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 -0.2 N.D. -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 -0.4 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.1 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.8 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.5 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 N.D. -0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.5 -0.1 N.D. +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.1 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.4 N.D. +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.2 N.D. +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.1 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.2 N.D. -0.2 N.D.
growth on ypd YPD +0.0 +0.0 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.3 N.D. +0.1 N.D.
growth on oleic acid YNB Glucose -0.0 -0.1 N.D. +0.3 N.D.
auxotrophy Dropout Complete +0.1 -0.3 N.D. +0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.2 N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.2 N.D. +0.5 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.3 N.D. -0.1 N.D.
auxotrophy YNB Glucose +0.5 -0.4 N.D. +0.5 N.D.
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