Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13486 and 13487 are separated by 550 nucleotides13487 and 13488 are separated by 879 nucleotides13488 and 13489 are separated by 351 nucleotides13489 and 13490 are separated by 442 nucleotides mRNA_5118: 13486 - K05349 bglX beta-glucosidase, at 1,800,635 to 1,803,494 13486 mRNA_5119: 13487 - HMMPfam-LysM domain-PF01476,SMART-Lysin motif-SM00257,SUPERFAMILY--SSF54106, at 1,804,045 to 1,805,977 13487 mRNA_5120: 13488 - K11279 NAP1L1, NRP nucleosome assembly protein 1-like 1, at 1,806,857 to 1,808,752 13488 mRNA_5121: 13489 - K01137 GNS N-acetylglucosamine-6-sulfatase, at 1,809,104 to 1,811,514 13489 mRNA_5122: 13490 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,811,957 to 1,814,516 13490
Group Condition mRNA_5118 mRNA_5119 mRNA_5120 mRNA_5121 mRNA_5122
13486 13487 13488 13489 13490
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.4 N.D. -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.1 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.3 N.D. -0.4 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 -1.0 N.D. -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.0 N.D. +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.0 N.D. -0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.0 N.D. -0.4 +0.4
growth on ypd YPD -0.1 +0.0 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -0.2 N.D. +0.6 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.2 N.D. +0.2 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.2 N.D. +0.5 +0.4
auxotrophy Dropout Complete -0.1 +0.6 N.D. -0.3 +0.0
growth on oleic acid YNB Glucose +0.1 -0.1 N.D. +0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 N.D. -0.1 +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -0.4 N.D. +0.1 +0.4
auxotrophy YNB Glucose +0.2 +0.3 N.D. -0.2 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.5 N.D. -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.7 N.D. -0.2 +0.1
growth on oleic acid YNB Oleic Acid -0.1 +0.5 N.D. +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.3 N.D. +0.5 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.3 N.D. +0.6 +0.2
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