Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13487 and 13488 are separated by 879 nucleotides13488 and 13489 are separated by 351 nucleotides13489 and 13490 are separated by 442 nucleotides13490 and 13491 are separated by 515 nucleotides mRNA_5119: 13487 - HMMPfam-LysM domain-PF01476,SMART-Lysin motif-SM00257,SUPERFAMILY--SSF54106, at 1,804,045 to 1,805,977 13487 mRNA_5120: 13488 - K11279 NAP1L1, NRP nucleosome assembly protein 1-like 1, at 1,806,857 to 1,808,752 13488 mRNA_5121: 13489 - K01137 GNS N-acetylglucosamine-6-sulfatase, at 1,809,104 to 1,811,514 13489 mRNA_5122: 13490 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,811,957 to 1,814,516 13490 mRNA_5123: 13491 - K14826 FPR3_4 FK506-binding nuclear protein, at 1,815,032 to 1,816,842 13491
Group Condition mRNA_5119 mRNA_5120 mRNA_5121 mRNA_5122 mRNA_5123
13487 13488 13489 13490 13491
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 N.D. -0.1 +0.2 -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.0 +0.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.1 +0.4 -0.5
growth on ypd YPD +0.0 N.D. +0.2 +0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.1 -0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. +0.1 +0.4 -0.0
growth on ricinoleic acid YNB Glucose +0.0 N.D. -0.2 +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.2 -0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.2 -0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. -0.4 +0.5 +0.6
growth on oleic acid YNB Glucose -0.1 N.D. +0.2 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. +0.6 +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. +0.5 +0.4 +0.1
auxotrophy YNB Glucose +0.3 N.D. -0.2 +0.4 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.4 +0.4 +0.9
auxotrophy Dropout Complete +0.6 N.D. -0.3 +0.0 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.5 +0.3 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. -0.1 +0.2 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.7 N.D. -0.2 +0.1 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.6 +0.4 +0.4
growth on oleic acid YNB Oleic Acid +0.5 N.D. +0.4 +0.0 +0.6
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