Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13488 and 13489 are separated by 351 nucleotides13489 and 13490 are separated by 442 nucleotides13490 and 13491 are separated by 515 nucleotides13491 and 13492 are separated by 149 nucleotides mRNA_5120: 13488 - K11279 NAP1L1, NRP nucleosome assembly protein 1-like 1, at 1,806,857 to 1,808,752 13488 mRNA_5121: 13489 - K01137 GNS N-acetylglucosamine-6-sulfatase, at 1,809,104 to 1,811,514 13489 mRNA_5122: 13490 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,811,957 to 1,814,516 13490 mRNA_5123: 13491 - K14826 FPR3_4 FK506-binding nuclear protein, at 1,815,032 to 1,816,842 13491 mRNA_5124: 13492 - K13248 PHOSPHO2 pyridoxal phosphate phosphatase PHOSPHO2, at 1,816,992 to 1,818,246 13492
Group Condition mRNA_5120 mRNA_5121 mRNA_5122 mRNA_5123 mRNA_5124
13488 13489 13490 13491 13492
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 +0.2 -0.7 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.4 -0.5 N.D.
growth on ypd YPD N.D. +0.2 +0.1 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.0 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.1 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.1 +0.0 N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.3 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.1 +0.0 N.D.
auxotrophy Dropout Complete N.D. -0.3 +0.0 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 +0.2 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.4 -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 +0.1 +0.5 N.D.
auxotrophy YNB Glucose N.D. -0.2 +0.4 +0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.2 +0.5 N.D.
growth on oleic acid YNB Glucose N.D. +0.2 +0.2 +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 +0.5 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.3 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 +0.4 +0.9 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.4 +0.0 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.5 +0.4 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 +0.4 +0.4 N.D.
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