Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13490 and 13491 are separated by 515 nucleotides13491 and 13492 are separated by 149 nucleotides13492 and 13493 are separated by 177 nucleotides13493 and 13494 are separated by 123 nucleotides mRNA_5122: 13490 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,811,957 to 1,814,516 13490 mRNA_5123: 13491 - K14826 FPR3_4 FK506-binding nuclear protein, at 1,815,032 to 1,816,842 13491 mRNA_5124: 13492 - K13248 PHOSPHO2 pyridoxal phosphate phosphatase PHOSPHO2, at 1,816,992 to 1,818,246 13492 mRNA_5125: 13493 - Interpro Protein of unknown function (DUF3431), at 1,818,424 to 1,820,541 13493 mRNA_5126: 13494 - Hypothetical Protein, at 1,820,665 to 1,821,231 13494
Group Condition mRNA_5122 mRNA_5123 mRNA_5124 mRNA_5125 mRNA_5126
13490 13491 13492 13493 13494
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.7 N.D. -1.4 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.1 N.D. -2.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.4 N.D. -0.8 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 N.D. -0.7 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.5 N.D. +0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 N.D. -0.1 -0.1
growth on ypd YPD +0.1 -0.4 N.D. -0.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 N.D. -0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.4 N.D. -0.9 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.0 N.D. -0.1 +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.0 N.D. -0.5 +0.8
growth on oleic acid YNB Glucose +0.2 +0.2 N.D. +0.5 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.5 N.D. +0.5 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.5 N.D. +0.3 +0.6
growth on oleic acid YNB Oleic Acid +0.0 +0.6 N.D. +0.9 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 N.D. +0.5 +1.1
growth on ricinoleic acid YNB Glucose +0.3 +0.1 N.D. +0.3 +1.3
auxotrophy YNB Glucose +0.4 +0.4 N.D. +0.6 +0.8
auxotrophy Dropout Complete +0.0 +0.7 N.D. +0.8 +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.9 N.D. +1.0 +1.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.6 N.D. +1.4 +1.5
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