Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13494 and 13495 are separated by 287 nucleotides13495 and 13496 are separated by 658 nucleotides13496 and 13497 are separated by 134 nucleotides13497 and 13498 are separated by 662 nucleotides mRNA_5126: 13494 - Hypothetical Protein, at 1,820,665 to 1,821,231 13494 mRNA_5127: 13495 - K07941 ARF6 ADP-ribosylation factor 6, at 1,821,519 to 1,822,822 13495 mRNA_5128: 13496 - K08342 ATG4 cysteine protease ATG4, at 1,823,481 to 1,827,071 13496 mRNA_5129: 13497 - Hypothetical Protein, at 1,827,206 to 1,828,600 13497 mRNA_5130: 13498 - Hypothetical Protein, at 1,829,263 to 1,829,910 13498
Group Condition mRNA_5126 mRNA_5127 mRNA_5128 mRNA_5129 mRNA_5130
13494 13495 13496 13497 13498
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -1.5 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.8 N.D. -2.0 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.0 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. -0.8 +0.7 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. +0.3 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. -1.3 +0.7 N.D.
growth on oleic acid YNB Glucose +0.2 N.D. +0.1 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.1 -0.0 N.D.
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.1 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +1.2 N.D. -0.3 -0.7 N.D.
auxotrophy Dropout Complete +0.9 N.D. -0.3 -0.3 N.D.
growth on ypd YPD +0.4 N.D. +0.1 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.1 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. -0.2 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +1.1 N.D. -0.1 -0.5 N.D.
growth on ricinoleic acid YNB Glucose +1.3 N.D. -0.2 -0.6 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +1.5 N.D. -0.2 -0.7 N.D.
auxotrophy YNB Glucose +0.8 N.D. -0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.7 +1.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.2 +0.8 N.D.
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