Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13496 and 13497 are separated by 134 nucleotides13497 and 13498 are separated by 662 nucleotides13498 and 13499 are separated by 166 nucleotides13499 and 13500 are separated by 346 nucleotides mRNA_5128: 13496 - K08342 ATG4 cysteine protease ATG4, at 1,823,481 to 1,827,071 13496 mRNA_5129: 13497 - Hypothetical Protein, at 1,827,206 to 1,828,600 13497 mRNA_5130: 13498 - Hypothetical Protein, at 1,829,263 to 1,829,910 13498 mRNA_5131: 13499 - Hypothetical Protein, at 1,830,077 to 1,833,638 13499 mRNA_5132: 13500 - K03025 RPC6, POLR3F DNA-directed RNA polymerase III subunit RPC6, at 1,833,985 to 1,836,173 13500
Group Condition mRNA_5128 mRNA_5129 mRNA_5130 mRNA_5131 mRNA_5132
13496 13497 13498 13499 13500
facs on lipid accumulation Sorted No BODIPY Gate -1.5 +0.2 N.D. +0.3 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 +0.7 N.D. +0.0 -1.5
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.2 N.D. +0.2 -1.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.7 N.D. +0.3 -0.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.7 N.D. +0.4 -0.8
facs on lipid accumulation Sorted High BODIPY Gate -2.0 -0.1 N.D. +0.5 +0.5
auxotrophy Dropout Complete -0.3 -0.3 N.D. +0.1 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.5 N.D. -0.2 -0.0
growth on ricinoleic acid YNB Glucose -0.2 -0.6 N.D. +0.1 -0.2
growth on oleic acid YNB Glucose +0.1 -0.4 N.D. +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 N.D. -0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.4 N.D. -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.0 N.D. +0.4 -0.5
growth on ypd YPD +0.1 -0.2 N.D. +0.0 -0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.0 N.D. +0.1 +0.0
auxotrophy YNB Glucose -0.3 +0.1 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.3 +0.7 N.D. +0.1 +0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.6 N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.8 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 +1.4 N.D. +0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.3 N.D. +0.5 +0.1
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