Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13518 and 13519 are separated by 1563 nucleotides13519 and 13520 are separated by 575 nucleotides13520 and 13521 are separated by 225 nucleotides13521 and 13522 are separated by 747 nucleotides mRNA_5150: 13518 - Hypothetical Protein, at 20,329 to 21,379 13518 mRNA_5151: 13519 - HMMPfam-SUR7/PalI family-PF06687, at 22,943 to 24,220 13519 mRNA_5152: 13520 - HMMPfam-Domain of unknown function (DUF4336)-PF14234,SUPERFAMILY--SSF56281, at 24,796 to 26,164 13520 mRNA_5153: 13521 - HMMPfam-Secretory lipase-PF03583,PIRSF--PIRSF029171,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF53474, at 26,390 to 28,027 13521 mRNA_5154: 13522 - HMMPfam-Beta-lactamase superfamily domain-PF12706,SUPERFAMILY--SSF56281, at 28,775 to 30,256 13522
Group Condition mRNA_5150 mRNA_5151 mRNA_5152 mRNA_5153 mRNA_5154
13518 13519 13520 13521 13522
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.9 -0.0 -1.0 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.6 +0.2 +0.1 -0.7 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.2 -0.1 -0.4 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 -0.4 +0.2 +0.0 +0.5
growth on ypd YPD -0.4 +0.0 +0.3 +0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.4 +0.0 -0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.2 +0.3 +0.0 -0.1
auxotrophy Dropout Complete -0.0 +0.4 +0.4 +0.4 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.7 +0.1 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.5 +0.1 +0.4 +0.1
growth on ricinoleic acid YNB Glucose +0.2 +0.6 -0.1 +0.6 -0.2
auxotrophy YNB Glucose -0.2 +0.7 +0.4 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.3 +0.2 +0.7 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.7 +0.3 +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.5 +0.3 +0.6 +0.6
growth on oleic acid YNB Glucose +0.4 +0.5 +0.2 +0.6 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.7 +0.4 +0.8 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +1.0 +0.0 +0.8 +0.3
growth on oleic acid YNB Oleic Acid +0.1 +1.1 +0.2 +0.6 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +1.2 +0.2 +0.8 +0.9
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +1.9 +0.7 +0.7 +0.3
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