Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13519 and 13520 are separated by 575 nucleotides13520 and 13521 are separated by 225 nucleotides13521 and 13522 are separated by 747 nucleotides13522 and 13523 are separated by 461 nucleotides mRNA_5151: 13519 - HMMPfam-SUR7/PalI family-PF06687, at 22,943 to 24,220 13519 mRNA_5152: 13520 - HMMPfam-Domain of unknown function (DUF4336)-PF14234,SUPERFAMILY--SSF56281, at 24,796 to 26,164 13520 mRNA_5153: 13521 - HMMPfam-Secretory lipase-PF03583,PIRSF--PIRSF029171,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF53474, at 26,390 to 28,027 13521 mRNA_5154: 13522 - HMMPfam-Beta-lactamase superfamily domain-PF12706,SUPERFAMILY--SSF56281, at 28,775 to 30,256 13522 mRNA_5155: 13523 - BLAST unknown [Tremella fuciformis], at 30,718 to 32,151 13523
Group Condition mRNA_5151 mRNA_5152 mRNA_5153 mRNA_5154 mRNA_5155
13519 13520 13521 13522 13523
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 -0.0 -1.0 -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 -0.4 -0.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.2 +0.0 +0.5 -1.2
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 -0.7 -0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.0 -0.3 -0.1 +0.4
growth on ypd YPD +0.0 +0.3 +0.0 +0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.7 +0.1 +0.1 +0.3 +0.0
auxotrophy Dropout Complete +0.4 +0.4 +0.4 -0.4 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.3 +0.0 -0.1 +0.8
growth on ricinoleic acid YNB Glucose +0.6 -0.1 +0.6 -0.2 +0.3
growth on oleic acid YNB Glucose +0.5 +0.2 +0.6 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.2 +0.7 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.1 +0.4 +0.1 +0.7
auxotrophy YNB Glucose 75mM Methionine +0.7 +0.3 +0.5 +0.1 +0.7
auxotrophy YNB Glucose +0.7 +0.4 +0.4 +0.1 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.3 +0.6 +0.6 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 +0.4 +0.8 +0.3 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 +0.0 +0.8 +0.3 +1.0
growth on oleic acid YNB Oleic Acid +1.1 +0.2 +0.6 +0.6 +0.8
facs on lipid accumulation Sorted High BODIPY Gate +1.9 +0.7 +0.7 +0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +1.2 +0.2 +0.8 +0.9 +1.4
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