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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
13519 and 13520 are separated by 575 nucleotides
13520 and 13521 are separated by 225 nucleotides
13521 and 13522 are separated by 747 nucleotides
13522 and 13523 are separated by 461 nucleotides
mRNA_5151: 13519 - HMMPfam-SUR7/PalI family-PF06687, at 22,943 to 24,220
13519
mRNA_5152: 13520 - HMMPfam-Domain of unknown function (DUF4336)-PF14234,SUPERFAMILY--SSF56281, at 24,796 to 26,164
13520
mRNA_5153: 13521 - HMMPfam-Secretory lipase-PF03583,PIRSF--PIRSF029171,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF53474, at 26,390 to 28,027
13521
mRNA_5154: 13522 - HMMPfam-Beta-lactamase superfamily domain-PF12706,SUPERFAMILY--SSF56281, at 28,775 to 30,256
13522
mRNA_5155: 13523 - BLAST unknown [Tremella fuciformis], at 30,718 to 32,151
13523
Group
Condition
mRNA
_5151
mRNA
_5152
mRNA
_5153
mRNA
_5154
mRNA
_5155
13519
13520
13521
13522
13523
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.9
-0.0
-1.0
-0.2
+0.1
ypd buoyancy
40 hrs Median Buoyancy
-0.2
-0.1
-0.4
-0.2
-0.4
ypd buoyancy
40 hrs No Buoyancy Separation
-0.4
+0.2
+0.0
+0.5
-1.2
ypd buoyancy
40 hrs High Buoyancy
+0.2
+0.1
-0.7
-0.2
-0.2
facs on lipid accumulation
Sorted No BODIPY Gate
+0.4
+0.0
-0.3
-0.1
+0.4
growth on ypd
YPD
+0.0
+0.3
+0.0
+0.2
+0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.7
+0.1
+0.1
+0.3
+0.0
auxotrophy
Dropout Complete
+0.4
+0.4
+0.4
-0.4
+0.4
auxotrophy
YNB Glucose 75mM Arginine
+0.2
+0.3
+0.0
-0.1
+0.8
growth on ricinoleic acid
YNB Glucose
+0.6
-0.1
+0.6
-0.2
+0.3
growth on oleic acid
YNB Glucose
+0.5
+0.2
+0.6
+0.3
+0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.3
+0.2
+0.7
+0.3
+0.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.5
+0.1
+0.4
+0.1
+0.7
auxotrophy
YNB Glucose 75mM Methionine
+0.7
+0.3
+0.5
+0.1
+0.7
auxotrophy
YNB Glucose
+0.7
+0.4
+0.4
+0.1
+0.7
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.5
+0.3
+0.6
+0.6
+0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.7
+0.4
+0.8
+0.3
+0.6
growth on ricinoleic acid
YNB Methylricinoleic Acid
+1.0
+0.0
+0.8
+0.3
+1.0
growth on oleic acid
YNB Oleic Acid
+1.1
+0.2
+0.6
+0.6
+0.8
facs on lipid accumulation
Sorted High BODIPY Gate
+1.9
+0.7
+0.7
+0.3
+0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
+1.2
+0.2
+0.8
+0.9
+1.4
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