Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13520 and 13521 are separated by 225 nucleotides13521 and 13522 are separated by 747 nucleotides13522 and 13523 are separated by 461 nucleotides13523 and 13524 are separated by 812 nucleotides mRNA_5152: 13520 - HMMPfam-Domain of unknown function (DUF4336)-PF14234,SUPERFAMILY--SSF56281, at 24,796 to 26,164 13520 mRNA_5153: 13521 - HMMPfam-Secretory lipase-PF03583,PIRSF--PIRSF029171,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF53474, at 26,390 to 28,027 13521 mRNA_5154: 13522 - HMMPfam-Beta-lactamase superfamily domain-PF12706,SUPERFAMILY--SSF56281, at 28,775 to 30,256 13522 mRNA_5155: 13523 - BLAST unknown [Tremella fuciformis], at 30,718 to 32,151 13523 mRNA_5156: 13524 - K00108 betA, CHDH choline dehydrogenase, at 32,964 to 35,897 13524
Group Condition mRNA_5152 mRNA_5153 mRNA_5154 mRNA_5155 mRNA_5156
13520 13521 13522 13523 13524
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -1.0 -0.2 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.4 -0.2 -0.4 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.7 -0.2 -0.2 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.0 +0.5 -1.2 +0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.3 -0.1 +0.4 -0.0
growth on ypd YPD +0.3 +0.0 +0.2 +0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 +0.3 +0.0 +0.2
growth on ricinoleic acid YNB Glucose -0.1 +0.6 -0.2 +0.3 +0.1
auxotrophy Dropout Complete +0.4 +0.4 -0.4 +0.4 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.0 -0.1 +0.8 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.4 +0.1 +0.7 +0.0
growth on oleic acid YNB Glucose +0.2 +0.6 +0.3 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.7 +0.3 +0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.5 +0.1 +0.7 +0.2
auxotrophy YNB Glucose +0.4 +0.4 +0.1 +0.7 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.7 +0.7 +0.3 +0.0 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.8 +0.3 +1.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.6 +0.6 +0.6 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.8 +0.3 +0.6 +0.2
growth on oleic acid YNB Oleic Acid +0.2 +0.6 +0.6 +0.8 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.8 +0.9 +1.4 -0.1
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