Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13521 and 13522 are separated by 747 nucleotides13522 and 13523 are separated by 461 nucleotides13523 and 13524 are separated by 812 nucleotides13524 and 13525 are separated by 420 nucleotides mRNA_5153: 13521 - HMMPfam-Secretory lipase-PF03583,PIRSF--PIRSF029171,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF53474, at 26,390 to 28,027 13521 mRNA_5154: 13522 - HMMPfam-Beta-lactamase superfamily domain-PF12706,SUPERFAMILY--SSF56281, at 28,775 to 30,256 13522 mRNA_5155: 13523 - BLAST unknown [Tremella fuciformis], at 30,718 to 32,151 13523 mRNA_5156: 13524 - K00108 betA, CHDH choline dehydrogenase, at 32,964 to 35,897 13524 mRNA_5157: 13525 - K11976 RNF216 TRIAD3 E3 ubiquitin-protein ligase RNF216, at 36,318 to 38,711 13525
Group Condition mRNA_5153 mRNA_5154 mRNA_5155 mRNA_5156 mRNA_5157
13521 13522 13523 13524 13525
ypd buoyancy 40 hrs High Buoyancy -0.7 -0.2 -0.2 -0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.2 +0.1 -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.4 -0.2 -0.4 -0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.5 -1.2 +0.5 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.1 +0.4 -0.0 +0.3
auxotrophy Dropout Complete +0.4 -0.4 +0.4 +0.0 -0.0
growth on ypd YPD +0.0 +0.2 +0.2 -0.0 +0.1
growth on ricinoleic acid YNB Glucose +0.6 -0.2 +0.3 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.1 +0.8 -0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.3 +0.0 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.1 +0.7 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.1 +0.7 +0.2 -0.2
growth on oleic acid YNB Glucose +0.6 +0.3 +0.2 +0.3 +0.2
auxotrophy YNB Glucose +0.4 +0.1 +0.7 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.3 +0.3 +0.3 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.7 +0.3 +0.0 +0.1 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 +0.3 +0.6 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.6 +0.6 +0.2 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 +0.3 +1.0 -0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.6 +0.6 +0.8 +0.3 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.8 +0.9 +1.4 -0.1 +0.3
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