Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13531 and 13532 are separated by 426 nucleotides13532 and 13533 are separated by 504 nucleotides13533 and 13534 are separated by 648 nucleotides13534 and 13535 are separated by 561 nucleotides mRNA_5163: 13531 - Hypothetical Protein, at 48,190 to 49,744 13531 mRNA_5164: 13532 - HMMPfam-Beta-lactamase superfamily domain-PF12706,SMART-Metallo-beta-lactamase superfamily-SM00849,SUPERFAMILY--SSF56281, at 50,171 to 51,821 13532 mRNA_5165: 13533 - Hypothetical Protein, at 52,326 to 52,658 13533 mRNA_5166: 13534 - KOG0590 Checkpoint kinase and related serine/threonine protein kinases, at 53,307 to 58,220 13534 mRNA_5167: 13535 - Hypothetical Protein, at 58,782 to 59,470 13535
Group Condition mRNA_5163 mRNA_5164 mRNA_5165 mRNA_5166 mRNA_5167
13531 13532 13533 13534 13535
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -1.3 +0.1 +0.0 -1.7
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.8 +0.0 +0.2 -0.9
growth on oleic acid YNB Oleic Acid +0.3 +0.2 -0.0 +0.1 -1.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.1 -0.1 +0.0 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.5 -0.1 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.5 +0.0 -0.1 -0.0
growth on ricinoleic acid YNB Glucose -0.0 -0.2 +0.0 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.5 +0.0 +0.6 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +0.0 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.2 +0.1 -0.2 +0.1
growth on ypd YPD +0.1 +0.4 +0.0 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.1 +0.0 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.5 +0.1 +0.8 -0.4
auxotrophy Dropout Complete +0.3 -0.6 +0.0 +0.4 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.5 -0.1 +0.4 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.3 -0.0 +0.5 +0.4
growth on oleic acid YNB Glucose +0.2 +0.3 -0.0 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.0 +0.3 +0.5 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.3 -0.0 +0.2 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.3 +0.0 +0.1 +0.6
auxotrophy YNB Glucose +0.3 -0.5 -0.1 +0.6 +1.0
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