Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13534 and 13535 are separated by 561 nucleotides13535 and 13536 are separated by 1463 nucleotides13536 and 13537 are separated by 234 nucleotides13537 and 13538 are separated by 449 nucleotides mRNA_5166: 13534 - KOG0590 Checkpoint kinase and related serine/threonine protein kinases, at 53,307 to 58,220 13534 mRNA_5167: 13535 - Hypothetical Protein, at 58,782 to 59,470 13535 mRNA_5168: 13536 - SUPERFAMILY--SSF46955, at 60,934 to 62,324 13536 mRNA_5169: 13537 - Hypothetical Protein, at 62,559 to 64,260 13537 mRNA_5170: 13538 - Hypothetical Protein, at 64,710 to 65,306 13538
Group Condition mRNA_5166 mRNA_5167 mRNA_5168 mRNA_5169 mRNA_5170
13534 13535 13536 13537 13538
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -1.7 +0.0 -1.2 -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.9 +0.6 -0.8 -1.7
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.5 -0.2 +0.2 -1.4
growth on oleic acid YNB Oleic Acid +0.1 -1.4 -0.0 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.0 +0.4 -0.3 -0.5
auxotrophy Dropout Complete +0.4 +0.5 -0.1 -0.2 -0.9
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.0 +0.4 -0.3 -0.3
growth on ricinoleic acid YNB Glucose +0.1 -0.1 +0.2 -0.1 -0.3
growth on oleic acid YNB Glucose +0.3 +0.1 +0.2 +0.0 -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.4 +0.5 -0.3 -0.9
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.5 +0.3 +0.0 -1.3
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.4 +0.2 -0.0 -1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 -0.4 +0.3 -0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +0.3 -0.2 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.6 -0.3 -0.1 +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.1 -0.2 -0.0 +1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 -0.0 +0.6 +0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 +0.4 -0.0 +1.0
growth on ypd YPD +0.0 -0.3 +0.3 -0.2 +1.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 -0.3 +0.7 -0.1 +0.6
auxotrophy YNB Glucose +0.6 +1.0 +0.5 -0.1 -0.3
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