Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13551 and 13552 are separated by 1043 nucleotides13552 and 13553 are separated by 1477 nucleotides13553 and 13554 are separated by 1090 nucleotides13554 and 13555 are separated by 690 nucleotides mRNA_5183: 13551 - K02924 RP-L39e, RPL39 large subunit ribosomal protein L39e, at 93,240 to 93,967 13551 mRNA_5184: 13552 - K03017 RPB9, POLR2I DNA-directed RNA polymerase II subunit RPB9, at 95,011 to 95,960 13552 mRNA_5185: 13553 - K01210 E3.2.1.58 glucan 1,3-beta-glucosidase, at 97,438 to 100,447 13553 mRNA_5186: 13554 - KOG0023 Alcohol dehydrogenase, class V, at 101,538 to 103,195 13554 mRNA_5187: 13555 - KOG2533 Permease of the major facilitator superfamily, at 103,886 to 106,875 13555
Group Condition mRNA_5183 mRNA_5184 mRNA_5185 mRNA_5186 mRNA_5187
13551 13552 13553 13554 13555
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.3 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.3 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.4 -0.2
auxotrophy YNB Glucose N.D. N.D. N.D. +0.0 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 -0.1
auxotrophy Dropout Complete N.D. N.D. N.D. +0.1 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.1 -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.4 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.5 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.1 -0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.0 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.3 -0.3
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.4 -0.3
growth on ypd YPD N.D. N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.3 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.2 +0.3
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