Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13554 and 13555 are separated by 690 nucleotides13555 and 13556 are separated by 792 nucleotides13556 and 13557 are separated by 615 nucleotides13557 and 13558 are separated by 385 nucleotides mRNA_5186: 13554 - KOG0023 Alcohol dehydrogenase, class V, at 101,538 to 103,195 13554 mRNA_5187: 13555 - KOG2533 Permease of the major facilitator superfamily, at 103,886 to 106,875 13555 mRNA_5188: 13556 - HMMPfam-Protein of unknown function (DUF3421)-PF11901,SMART-Repeats found in Drosophila proteins.-SM00696, at 107,668 to 108,466 13556 mRNA_5189: 13557 - K14290 XPO1, CRM1 exportin-1, at 109,082 to 113,752 13557 mRNA_5190: 13558 - KOG4157 beta-1,6-N-acetylglucosaminyltransferase, contains WSC domain, at 114,138 to 116,063 13558
Group Condition mRNA_5186 mRNA_5187 mRNA_5188 mRNA_5189 mRNA_5190
13554 13555 13556 13557 13558
auxotrophy Dropout Complete +0.1 -0.5 -0.7 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.4 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.1 -0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.2 -0.2 N.D. N.D.
auxotrophy YNB Glucose +0.0 -0.5 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.4 -0.0 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.8 +0.6 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.0 -0.3 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.5 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.3 -0.5 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.3 -0.5 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.2 -0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.4 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 -0.1 +0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 +0.1 +0.2 N.D. N.D.
growth on ypd YPD +0.1 -0.1 -0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.0 +0.3 N.D. N.D.
growth on oleic acid YNB Glucose +0.4 -0.3 -0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 +0.2 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.3 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.2 +0.2 N.D. N.D.
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