Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13559 and 13560 are separated by 121 nucleotides13560 and 13561 are separated by 458 nucleotides13561 and 13562 are separated by 891 nucleotides13562 and 13563 are separated by 697 nucleotides mRNA_5191: 13559 - KOG4826 C-8,7 sterol isomerase, at 116,613 to 117,622 13559 mRNA_5192: 13560 - KOG2814 Transcription coactivator complex, P50 component (LigT RNA ligase/phosphodiesterase family), at 117,744 to 118,664 13560 mRNA_5193: 13561 - KOG0253 Synaptic vesicle transporter SV2 (major facilitator superfamily), at 119,123 to 121,882 13561 mRNA_5194: 13562 - K00002 AKR1A1, adh alcohol dehydrogenase (NADP+), at 122,774 to 124,635 13562 mRNA_5195: 13563 - KOG0814 Glyoxylase, at 125,333 to 126,456 13563
Group Condition mRNA_5191 mRNA_5192 mRNA_5193 mRNA_5194 mRNA_5195
13559 13560 13561 13562 13563
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.9 +0.6 +0.1 -3.8
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.3 +0.2 -0.4 -1.7
growth on oleic acid YNB Oleic Acid +0.1 -1.5 +0.2 +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.4 -0.4 +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +1.0 +0.7 -0.2 -2.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.0 +0.1 -0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.4 +0.1 -0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.3 +0.2 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.4 -0.7 +0.3 +0.5
auxotrophy Dropout Complete -0.2 -0.7 +0.1 +0.5 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.1 -0.7 +0.7 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.6 -0.1 -0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +1.5 -0.9 +0.4 -0.6
growth on ypd YPD -0.4 +0.2 -0.1 +0.3 +0.4
growth on oleic acid YNB Glucose +0.0 -1.0 +0.4 +0.6 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.7 +0.3 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.5 +0.1 -0.3 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.3 +0.2 +0.6 -0.1
auxotrophy YNB Glucose +0.1 -0.5 +0.2 +0.5 +0.1
growth on ricinoleic acid YNB Glucose -0.0 -0.2 -0.1 +0.5 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.5 +0.4 +0.5 +0.3
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