Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13560 and 13561 are separated by 458 nucleotides13561 and 13562 are separated by 891 nucleotides13562 and 13563 are separated by 697 nucleotides13563 and 13564 are separated by 434 nucleotides mRNA_5192: 13560 - KOG2814 Transcription coactivator complex, P50 component (LigT RNA ligase/phosphodiesterase family), at 117,744 to 118,664 13560 mRNA_5193: 13561 - KOG0253 Synaptic vesicle transporter SV2 (major facilitator superfamily), at 119,123 to 121,882 13561 mRNA_5194: 13562 - K00002 AKR1A1, adh alcohol dehydrogenase (NADP+), at 122,774 to 124,635 13562 mRNA_5195: 13563 - KOG0814 Glyoxylase, at 125,333 to 126,456 13563 mRNA_5196: 13564 - K17218 sqr sulfide-quinone oxidoreductase, at 126,891 to 128,997 13564
Group Condition mRNA_5192 mRNA_5193 mRNA_5194 mRNA_5195 mRNA_5196
13560 13561 13562 13563 13564
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 +0.6 +0.1 -3.8 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.2 -0.4 -1.7 -0.2
growth on oleic acid YNB Oleic Acid -1.5 +0.2 +0.3 +0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +1.0 +0.7 -0.2 -2.4 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.4 +0.1 +0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.7 +0.3 +0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.7 +0.7 +0.2 -0.5
auxotrophy Dropout Complete -0.7 +0.1 +0.5 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.1 -0.3 +0.0 -0.6
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.1 -0.3 -0.4 -0.1
auxotrophy YNB Glucose -0.5 +0.2 +0.5 +0.1 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.2 -0.2 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.1 -0.2 -0.2 +0.5
growth on oleic acid YNB Glucose -1.0 +0.4 +0.6 +0.3 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.5 +0.4 +0.5 +0.3 -0.5
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.2 +0.6 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 -0.1 -0.2 -0.1 +0.1
growth on ricinoleic acid YNB Glucose -0.2 -0.1 +0.5 +0.4 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +1.5 -0.9 +0.4 -0.6 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.7 +0.3 -0.1 -0.2 +0.1
growth on ypd YPD +0.2 -0.1 +0.3 +0.4 +0.1
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