Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13561 and 13562 are separated by 891 nucleotides13562 and 13563 are separated by 697 nucleotides13563 and 13564 are separated by 434 nucleotides13564 and 13565 are separated by 117 nucleotides mRNA_5193: 13561 - KOG0253 Synaptic vesicle transporter SV2 (major facilitator superfamily), at 119,123 to 121,882 13561 mRNA_5194: 13562 - K00002 AKR1A1, adh alcohol dehydrogenase (NADP+), at 122,774 to 124,635 13562 mRNA_5195: 13563 - KOG0814 Glyoxylase, at 125,333 to 126,456 13563 mRNA_5196: 13564 - K17218 sqr sulfide-quinone oxidoreductase, at 126,891 to 128,997 13564 mRNA_5197: 13565 - Hypothetical Protein, at 129,115 to 130,730 13565
Group Condition mRNA_5193 mRNA_5194 mRNA_5195 mRNA_5196 mRNA_5197
13561 13562 13563 13564 13565
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 +0.1 -3.8 -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.4 -1.7 -0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.7 -0.2 -2.4 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 +0.4 -0.6 -0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.3 -0.4 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.3 +0.0 -0.6 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 +0.7 +0.2 -0.5 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.1 +0.2 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 -0.1 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.1 -0.2 +0.1 -0.2
auxotrophy Dropout Complete +0.1 +0.5 -0.0 -0.2 -0.2
auxotrophy YNB Glucose +0.2 +0.5 +0.1 -0.4 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 +0.3 +0.5 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 -0.2 +0.5 +0.2
growth on ypd YPD -0.1 +0.3 +0.4 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.2 +0.1 +0.2 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.6 -0.1 -0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.1 +0.5 +0.4 -0.2 -0.0
growth on oleic acid YNB Oleic Acid +0.2 +0.3 +0.1 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.5 +0.3 -0.5 -0.0
growth on oleic acid YNB Glucose +0.4 +0.6 +0.3 -0.2 -0.0
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