Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13566 and 13567 are separated by 108 nucleotides13567 and 13568 are separated by 303 nucleotides13568 and 13569 are separated by 769 nucleotides13569 and 13570 are separated by 307 nucleotides mRNA_5198: 13566 - KOG2614 Kynurenine 3-monooxygenase and related flavoprotein monooxygenases, at 131,412 to 133,498 13566 mRNA_5199: 13567 - K01210 E3.2.1.58 glucan 1,3-beta-glucosidase, at 133,607 to 137,030 13567 mRNA_5200: 13568 - Hypothetical Protein, at 137,334 to 138,235 13568 mRNA_5201: 13569 - K02304 MET8 precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase, at 139,005 to 139,988 13569 mRNA_5202: 13570 - K02874 RP-L14, MRPL14, rplN large subunit ribosomal protein L14, at 140,296 to 141,028 13570
Group Condition mRNA_5198 mRNA_5199 mRNA_5200 mRNA_5201 mRNA_5202
13566 13567 13568 13569 13570
auxotrophy YNB Glucose +0.4 -0.2 -0.3 -3.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.1 -0.2 -2.7 N.D.
growth on oleic acid YNB Glucose -0.1 -0.4 +0.0 -2.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.1 -0.3 -2.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.6 +0.4 +0.1 -3.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.7 -0.0 -2.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.5 -0.2 -1.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.6 +0.1 +0.0 -2.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.2 +0.1 -1.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.3 -0.1 -1.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.1 -0.1 -0.8 N.D.
growth on oleic acid YNB Oleic Acid +0.1 +0.2 +0.1 -0.7 N.D.
growth on ypd YPD +0.0 +0.0 +0.4 -0.8 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.2 +0.1 -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.3 -0.2 +0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.2 +0.6 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.9 -0.1 -0.4 N.D.
auxotrophy Dropout Complete +0.1 +0.1 -0.3 +1.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 +0.3 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.6 -0.3 +0.7 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.4 +0.1 +0.7 N.D.
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