Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13569 and 13570 are separated by 307 nucleotides13570 and 13571 are separated by 383 nucleotides13571 and 13572 are separated by 401 nucleotides13572 and 13573 are separated by 1211 nucleotides mRNA_5201: 13569 - K02304 MET8 precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase, at 139,005 to 139,988 13569 mRNA_5202: 13570 - K02874 RP-L14, MRPL14, rplN large subunit ribosomal protein L14, at 140,296 to 141,028 13570 mRNA_5203: 13571 - K06709 KDM4, JMJD2, JHDM3 jumonji domain-containing protein 2, at 141,412 to 145,870 13571 mRNA_5204: 13572 - Hypothetical Protein, at 146,272 to 147,091 13572 mRNA_5205: 13573 - KOG0001 Ubiquitin and ubiquitin-like proteins, at 148,303 to 148,964 13573
Group Condition mRNA_5201 mRNA_5202 mRNA_5203 mRNA_5204 mRNA_5205
13569 13570 13571 13572 13573
facs on lipid accumulation Sorted High BODIPY Gate -3.4 N.D. +0.3 -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine -2.7 N.D. +0.3 -0.2 -0.4
growth on oleic acid YNB Glucose -2.1 N.D. +0.3 -0.2 -0.5
auxotrophy YNB Glucose -3.1 N.D. +0.3 +0.5 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.6 N.D. +0.1 -0.5 +0.9
growth on ricinoleic acid YNB Glucose -2.0 N.D. +0.1 +0.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. +0.3 -1.4 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -1.7 N.D. -0.1 +0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -2.3 N.D. +0.2 +1.1 -0.2
growth on oleic acid YNB Oleic Acid -0.7 N.D. +0.3 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.9 N.D. +0.1 +0.6 +0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. -0.2 +0.4 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -1.3 N.D. -0.0 +0.6 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. +0.1 -0.3 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 N.D. +0.2 -1.4 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.1 -0.1 +0.2
growth on ypd YPD -0.8 N.D. -0.2 +1.6 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. -0.0 +0.9 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.7 N.D. +0.0 +0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. +0.2 +0.8 +0.1
auxotrophy Dropout Complete +1.0 N.D. +0.3 +0.5 +0.0
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