Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13573 and 13574 are separated by 913 nucleotides13574 and 13575 are separated by 1015 nucleotides13575 and 13576 are separated by 35 nucleotides13576 and 13577 are separated by 445 nucleotides mRNA_5205: 13573 - KOG0001 Ubiquitin and ubiquitin-like proteins, at 148,303 to 148,964 13573 mRNA_5206: 13574 - HMMPfam-Ubiquitin family-PF00240,ProSiteProfiles-Ubiquitin domain profile.-PS50053,SMART-Ubiquitin homologues-SM00213,SUPERFAMILY--SSF54236, at 149,878 to 151,168 13574 mRNA_5207: 13575 - KOG0001 Ubiquitin and ubiquitin-like proteins, at 152,184 to 152,471 13575 mRNA_5208: 13576 - Hypothetical Protein, at 152,507 to 152,935 13576 mRNA_5209: 13577 - K03257 EIF4A translation initiation factor 4A, at 153,381 to 155,810 13577
Group Condition mRNA_5205 mRNA_5206 mRNA_5207 mRNA_5208 mRNA_5209
13573 13574 13575 13576 13577
growth on oleic acid YNB Glucose -0.5 +0.0 N.D. -0.3 N.D.
growth on ricinoleic acid YNB Glucose -0.1 -0.5 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.4 N.D. -0.6 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.3 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.6 N.D. -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.5 N.D. +0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.0 +0.0 N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.1 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 -0.1 N.D. -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.0 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.1 N.D. -0.3 N.D.
auxotrophy YNB Glucose -0.0 -0.3 N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.8 +0.1 N.D. -0.7 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.0 N.D. +0.2 N.D.
growth on ypd YPD +0.0 -0.2 N.D. +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 +0.0 N.D. -0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.1 N.D. +0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.2 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.3 N.D. +0.2 N.D.
auxotrophy Dropout Complete +0.0 +0.2 N.D. +0.4 N.D.
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