Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13574 and 13575 are separated by 1015 nucleotides13575 and 13576 are separated by 35 nucleotides13576 and 13577 are separated by 445 nucleotides13577 and 13578 are separated by 925 nucleotides mRNA_5206: 13574 - HMMPfam-Ubiquitin family-PF00240,ProSiteProfiles-Ubiquitin domain profile.-PS50053,SMART-Ubiquitin homologues-SM00213,SUPERFAMILY--SSF54236, at 149,878 to 151,168 13574 mRNA_5207: 13575 - KOG0001 Ubiquitin and ubiquitin-like proteins, at 152,184 to 152,471 13575 mRNA_5208: 13576 - Hypothetical Protein, at 152,507 to 152,935 13576 mRNA_5209: 13577 - K03257 EIF4A translation initiation factor 4A, at 153,381 to 155,810 13577 mRNA_5210: 13578 - KOG3221 Glycolipid transfer protein, at 156,736 to 157,976 13578
Group Condition mRNA_5206 mRNA_5207 mRNA_5208 mRNA_5209 mRNA_5210
13574 13575 13576 13577 13578
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. +0.3 N.D. -2.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.2 N.D. -2.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 N.D. -0.1 N.D. -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. +0.6 N.D. -1.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.4 N.D. -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -0.4 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. +0.1 N.D. -0.2
growth on oleic acid YNB Glucose +0.0 N.D. -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.7 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. -0.6 N.D. +0.9
growth on ricinoleic acid YNB Glucose -0.5 N.D. -0.2 N.D. +0.7
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.1 N.D. -0.0
auxotrophy YNB Glucose -0.3 N.D. +0.5 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. +0.2 N.D. +0.0
growth on ypd YPD -0.2 N.D. +0.4 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.3 N.D. +0.6
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.1 N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +0.2 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.1 N.D. +0.3
auxotrophy Dropout Complete +0.2 N.D. +0.4 N.D. +0.7
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