Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13580 and 13581 are separated by 624 nucleotides13581 and 13582 are separated by 194 nucleotides13582 and 13583 are separated by 146 nucleotides13583 and 13585 are separated by 315 nucleotides mRNA_5212: 13580 - K00624 E2.3.1.7 carnitine O-acetyltransferase, at 159,635 to 162,568 13580 mRNA_5213: 13581 - Hypothetical Protein, at 163,193 to 164,200 13581 mRNA_5214: 13582 - K03845 ALG3 alpha-1,3-mannosyltransferase, at 164,395 to 166,467 13582 mRNA_5215: 13583 - KOG1940 Zn-finger protein, at 166,614 to 167,435 13583 mRNA_5217: 13585 - Hypothetical Protein, at 167,751 to 168,514 13585
Group Condition mRNA_5212 mRNA_5213 mRNA_5214 mRNA_5215 mRNA_5217
13580 13581 13582 13583 13585
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.2 -0.5 -0.7 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.1 -1.3 +0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 -0.2 -0.3 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.1 -0.2 +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.1 +0.1 -0.3 +0.2
auxotrophy Dropout Complete +0.2 -0.0 -0.0 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.3 +0.1 +0.6 -0.5
auxotrophy YNB Glucose +0.2 -0.2 -0.1 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.4 -0.1 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.4 -0.1 +0.4 -0.3
growth on oleic acid YNB Oleic Acid -0.0 +0.4 +0.4 -0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 +0.0 -0.2 +0.2
growth on ricinoleic acid YNB Glucose +0.5 +0.1 -0.2 +0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 -0.2 +0.1 +0.4
growth on oleic acid YNB Glucose +0.3 +0.4 +0.3 -0.3 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 -0.2 +0.3 +0.6
growth on ypd YPD +0.4 -0.1 +0.1 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.4 -0.3 +0.2 +1.0
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.1 +0.2 +0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.2 -0.1 +1.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.2 -0.0 +0.8 +0.8
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